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| Status |
Public on Aug 26, 2009 |
| Title |
Gene expression profiles in rat lung following intratracheal instillation with C60 fullerene particles |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression profiling of the rat lung following intratracheal instillation with C60 fullerene particles was employed to gain insights into these molecular events.
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| Overall design |
Groups of nine-week-old male Wistar rats (n= 4-6 per group/ time point) were intratracheally instilled with C60 fullerene suspended in 0.4 ml distilled water including 0.8% Tween-80 as a single injection (0.1 mg, 0.2 mg (LD: low dose) and 1.0 mg (HD: high dose) C60 fullerene/ rat). Control groups received 0.8% Tween-80 (vehicle control). After intratracheal instillation treatment, rats were housed within polycarbonate cages at a controlled temperature of 22 °C with a chow diet ad libitum, and dissected at 3 days, 1 week, 1 month, 3 month, and 6 month post-instillation. Right lungs of anesthetized rats were perfused with physiological saline, excised, and used for DNA microarray analysis.
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| Web link |
https://doi.org/10.1016/j.tox.2010.05.004
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| |
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| Contributor(s) |
Fujita K, Iwahashi H |
| Citation(s) |
20471445 |
| |
| Submission date |
Aug 20, 2009 |
| Last update date |
Dec 10, 2024 |
| Contact name |
Katsuhide Fujita |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Advanced Industrial Science and Technology
|
| Department |
Research Institute of Science for Safety and Sustainability
|
| Street address |
16-1 Onogawa
|
| City |
Tsukuba |
| ZIP/Postal code |
3058569 |
| Country |
Japan |
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| Platforms (1) |
| GPL7294 |
Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Probe Name version) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA118349 |
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