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| Status |
Public on Oct 25, 2021 |
| Title |
RNA-seq using mouse Neuro-2A (N2A) neuroblastoma cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To identify high-confidence NMD targets in mouse N2A neuroblastoma cells, we used our established transcriptome-wide RNA sequencing (RNA-seq) methodologies. Through parallel analyses of RNA-seq upon UPF1-knockdown (KD) and RNA immunoprecipitation (RIP-seq) footprinting of p-UPF1-bound RNAs, we identified 1027 high-confidence neuronal NMD targets.
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| Overall design |
RNA profiles of N2A cells treated with control siRNA (siCtl; n=2) or Upf1 siRNA (siUpf1; n=3).
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| Contributor(s) |
Kurosaki T, Maquat LE |
| Citation(s) |
34784943 |
| BioProject |
PRJNA745214 |
| |
| Submission date |
Jul 14, 2021 |
| Last update date |
Nov 30, 2021 |
| Contact name |
Tatsuaki Kurosaki |
| E-mail(s) |
[email protected]
|
| Phone |
5852812657
|
| Organization name |
University of Rochester
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| Department |
Biochemistry and Biophysics
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| Lab |
Lynne Maquat lab
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| Street address |
601 Elmwood Avenue, Box 712
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| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL30028 |
illumina NovaSeq 6000 (Mus musculus) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE180137 |
NMD abnormalities during brain development in the Fmr1-knockout mouse model of fragile X syndrome |
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