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| Status |
Public on May 05, 2010 |
| Title |
Methylation status in human sperm |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
Genomic imprinting describes the expression of a subset of mammalian genes from one parental chromosome. The parent-of-origin specific expression of imprinted genes relies on DNA methylation of CpG-dinucleotides at differentially methylated regions (DMRs) during gametogenesis. We identified the paternally methylated DMR at human chromosome 2 near the imprinted ZDBF2 gene using a methylated-DNA immunoprecipitation-on-chip (meDIP-on-chip) method applied to DNA from sperm.
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| Overall design |
To analyze whether or not the GPR1-ZDBF2 DMR is conserved in human genome, methylation analysis of human sperm sample was performed using MeDIP and genome tiling array.
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| Contributor(s) |
Hiura H, Sugawara A, Ogawa H, John RM, Miyauchi N, Miyanari Y, Horiike T, Li Y, Yaegashi N, Sasaki H, Kono T, Arima T |
| Citation(s) |
20385583 |
| |
| Submission date |
Sep 20, 2009 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Hitoshi Hiura |
| E-mail(s) |
[email protected]
|
| Phone |
+81 22 717 7844
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| Organization name |
Tohoku University Graduate School of Medicine
|
| Department |
Obstetrics and Gynecology
|
| Street address |
2-1 Seiryo-cho, Aoba-ku
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| City |
Sendai |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
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| Platforms (1) |
| GPL9641 |
Agilent-014821 Human Whole Genome ChIP-on-Chip Set 244K - 4 of 25 (Probe Name version) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE21662 |
A tripartite paternally methylated region within the Gpr1-Zdbf2 imprinted domain on mouse chromosome 1 identified by meDIP-on-chip |
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| Relations |
| BioProject |
PRJNA129045 |
