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| Status |
Public on Oct 10, 2009 |
| Title |
Expression data from chicken bursal cells treated with stem cell antigen 2 |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
The differentiation and proliferation of chicken B cells rely on the antigens on the cell surface of bursal stromal cells. Chicken stem cell antigen 2 (SCA2) is localized on the surface of bursal cortical and medullary epithelial cells. The signals through SCA2 may regulate the expressions critical for the B cell development.
The altered expressions of bursal cells that were induced through SCA2-signaling were detailed via Affemetrix array experiments.
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| Overall design |
Bursa of 3 week old line 0 chickens were collected and manually teased to harvest the bursal cells. The bursal cells were spread on the monolayers of SCA2Flag-DF1 cells or control Vector-DF1 cells in triplicate. After growth in the mixture of Liebovitz’s L-15 and McCoy 5A media (1:1) supplemented with 4% FBS, penicillin, and streptomycin for overnight at 37 ºC in 5% CO2, the bursal cells were recovered by washes (DF1 cells are adhesive) followed by gentle centrifugation at 1000×g for 10 min. Total RNA was isolated using the absolutely RNA microprep kit.
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| Contributor(s) |
Mao W, Cheng H |
| Citation(s) |
19945479 |
| |
| Submission date |
Oct 09, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Weifeng Mao |
| E-mail(s) |
[email protected]
|
| Phone |
517 337 6626
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| Organization name |
Michigan State University
|
| Department |
Genetics Program
|
| Lab |
Hans Cheng
|
| Street address |
3606 E. Mount Hope Rd. USDA-ARS
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48823 |
| Country |
USA |
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| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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| Samples (6)
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| GSM461156 |
Bursal cells (control), biological rep 1 |
| GSM461157 |
Bursal cells (control), biological rep 2 |
| GSM461158 |
Bursal cells (control), biological rep 3 |
| GSM461159 |
Bursal cells induced by SCA2, biological rep 1 |
| GSM461160 |
Bursal cells induced by SCA2, biological rep 2 |
| GSM461161 |
Bursal cells induced by SCA2, biological rep 3 |
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| Relations |
| BioProject |
PRJNA118265 |
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