|
| Status |
Public on Oct 27, 2022 |
| Title |
An organoids-on-a-chip model of human ARPKD |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Microarray of kidney organoids derived from PKHD1 mutant human pluripotent stem cells.
|
| |
|
| Overall design |
Total RNA samples were prepared from PKHD1-/- and PKHD1+/- organoids in three different culture conditions: 1. flow, 2. static, or 3. forskolin (FK) on day 35. Gene expression was analyzed using 3D-Gene® Human 25k oligonucleotide microarrays. Sample preparation for array hybridization was carried out as described in the manufacturer's instructions.
|
| |
|
| Contributor(s) |
Miyoshi T, Hiratsuka K, Morizane R |
| Citation(s) |
36129975 |
| |
| Submission date |
Dec 06, 2021 |
| Last update date |
Oct 28, 2022 |
| Contact name |
Ryuji Morizane |
| E-mail(s) |
[email protected]
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Nephrology Division
|
| Street address |
149 13th Street
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13915 |
3D-Gene Human Oligo chip 25k V2.1 |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA786570 |
Less...
More...