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| Status |
Public on Jun 15, 2010 |
| Title |
Hyperactivation of PI3K promotes escape from hormone dependence in estrogen receptor-positive breast cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Hyperactivation of phosphatidylinositol-3 kinase (PI3K) promotes escape from hormone dependence in estrogen receptor-positive breast cancer.
A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. We used gene expression microarrays to identify genes and pathways that are commonly dysregulated in ER+ cell lines with acquired hormone-independent growth. MCF-7, ZR75-1, MDA-361, and HCC-1428 ER+, estrogen-responsive breast cancer cells were cultured under hormone-depleted conditions (10% DCC-FBS) for several months until sustainable hormone-independent cell populations emerged.
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| Overall design |
Parental and long-term estrogen-deprived (LTED) cells were treated with 10% dextran-coated charcoal-treated fetal bovine serum (DCC-FBS) x 24 hrs prior to RNA harvest for array analysis.
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| Contributor(s) |
Miller T, Arteaga C |
| Citation(s) |
20530877, 21346144 |
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| Submission date |
Dec 23, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Todd W Miller |
| E-mail(s) |
[email protected]
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| Organization name |
Vanderbilt Univ
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| Department |
Medicine
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| Street address |
2220 Pierce Ave, 777 PRB
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| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37174 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (24)
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| GSM490046 |
MCF-7/LTED, rep 1 |
| GSM490047 |
MCF-7/LTED, rep 2 |
| GSM490048 |
MCF-7/LTED, rep 3 |
| GSM490049 |
ZR75-1, rep 1 |
| GSM490050 |
ZR75-1, rep 2 |
| GSM490051 |
ZR75-1, rep 3 |
| GSM490052 |
ZR75-1/LTED, rep 1 |
| GSM490053 |
ZR75-1/LTED, rep 2 |
| GSM490054 |
ZR75-1/LTED, rep 3 |
| GSM490055 |
MDA-361, rep 1 |
| GSM490056 |
MDA-361, rep 2 |
| GSM490057 |
MDA-361, rep 3 |
| GSM490058 |
MDA-361/LTED, rep 1 |
| GSM490059 |
MDA-361/LTED, rep 2 |
| GSM490060 |
MDA-361/LTED, rep 3 |
| GSM490061 |
HCC-1428, rep 1 |
| GSM490062 |
HCC-1428, rep 2 |
| GSM490063 |
HCC-1428, rep 3 |
| GSM490064 |
HCC-1428/LTED, rep 1 |
| GSM490065 |
HCC-1428/LTED, rep 2 |
| GSM490066 |
HCC-1428/LTED, rep 3 |
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| Relations |
| BioProject |
PRJNA122527 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19639_RAW.tar |
116.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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