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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 23, 2022 |
| Title |
Developmental genome-wide occupancy analysis of bZIP transcription factor NRL uncovers the role of c-Jun in early differentiation of rod photoreceptors in the mammalian retina [CUT&RUN] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The basic motif-leucine zipper (bZIP) transcription factor NRL determines rod photoreceptor cell fate during retinal development, and its loss leads to cone-only retina in mice. NRL works synergistically with homeodomain protein Cone-Rod Homeobox and other regulatory factors to control the transcription of most genes associated with rod morphogenesis and functional maturation, which span over a period of several weeks in the mammalian retina. We predicted that NRL gradually establishes rod cell identity and function by temporal and dynamic regulation of stage-specific transcriptional targets. Therefore, we mapped the genomic occupancy of NRL at four stages of mouse photoreceptor differentiation by CUT&RUN analysis. Dynamics of NRL binding revealed concordance with the corresponding changes in transcriptome of the developing rods. Notably, we identified c-Jun proto-oncogene as one of the targets of NRL, which could bind to specific cis-elements in the c-Jun promoter and modulate its activity in HEK293 cells. Coimmunoprecipitation studies showed the association of NRL with c-Jun, also a bZIP protein, in transfected cells as well as in developing mouse retina. Additionally, shRNA-mediated knockdown of c-Jun in the mouse retina in vivo resulted in altered expression of almost 1000 genes, with reduced expression of phototransduction genes and many direct targets of NRL in rod photoreceptors. We propose that c-Jun-NRL heterodimers prime the NRL-directed transcriptional program in neonatal rod photoreceptors before high NRL expression suppresses c-Jun at later stages. Our study highlights a broader cooperation among cell-type restricted and widely expressed bZIP proteins, such as c-Jun, in specific spatiotemporal contexts during cellular differentiation.
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| Overall design |
CUT&RUN protocol (Skene, Henikoff et al. 2018) was performed using anti-Nrl antibody on 250,000 retinal cells per experiment at postnatal day P2, P4, P10, and P28. Experiments were performed in quadruplicate and were paried-end sequenced on an Illumina HiSeq 2500.
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| Contributor(s) |
Liang X, Brooks MJ, Swaroop A |
| Citation(s) |
35776116 |
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| Submission date |
Feb 24, 2022 |
| Last update date |
Sep 24, 2022 |
| Contact name |
Matthew J Brooks |
| E-mail(s) |
[email protected]
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| Phone |
301-443-4906
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| Organization name |
NIH
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| Department |
NEI
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| Lab |
NNRL
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| Street address |
6 Center Dr Bldg 6, Rm 303
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (15)
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| GSM5917164 |
retina, P4, NRL, 1 |
| GSM5917165 |
retina, P4, NRL, 2 |
| GSM5917166 |
retina, P4, NRL, 3 |
| GSM5917167 |
retina, P4, NRL, 4 |
| GSM5917168 |
retina, P10, NRL, 1 |
| GSM5917169 |
retina, P10, NRL, 2 |
| GSM5917170 |
retina, P10, NRL, 3 |
| GSM5917171 |
retina, P10, NRL, 4 |
| GSM5917172 |
retina, P28, NRL, 1 |
| GSM5917173 |
retina, P28, NRL, 2 |
| GSM5917174 |
retina, P28, NRL, 3 |
| GSM5917175 |
retina, P28, NRL, 4 |
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| This SubSeries is part of SuperSeries: |
| GSE197421 |
Developmental genome-wide occupancy analysis of bZIP transcription factor NRL uncovers the role of c-Jun in early differentiation of rod photoreceptors in the mammalian retina |
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| Relations |
| BioProject |
PRJNA810190 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE197420_P10_NRL_idr_peaks.tsv.gz |
480.7 Kb |
(ftp)(http) |
TSV |
| GSE197420_P28_NRL_idr_peaks.tsv.gz |
233.1 Kb |
(ftp)(http) |
TSV |
| GSE197420_P2_NRL_idr_peaks.tsv.gz |
85.7 Kb |
(ftp)(http) |
TSV |
| GSE197420_P4_NRL_idr_peaks.tsv.gz |
214.7 Kb |
(ftp)(http) |
TSV |
| GSE197420_RAW.tar |
1.3 Gb |
(http)(custom) |
TAR (of BW) |
| GSE197420_peak_quantitation.tsv.gz |
924.5 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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