|
| Status |
Public on Jun 15, 2022 |
| Title |
Comprehensive identification of RNA transcripts and construction of RNA networks in chronic obstructive pulmonary disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Here, we report a comprehensive analysis of dysregulated mRNAs, lncRNAs, miRNAs and circRNAs in peripheral blood of COPD patients with high-throughput RNA sequencing. We mapped about 50~80 million sequence reads per sample to the human genome. GSEA analysis showed that these dysregulated RNAs correlate with several critical biological processes such as RNA modification, and RNA metabolism. RT-qPCR (SYBR Green assays) with more clinical COPD samples were used for the validation of some dysregulated RNAs. We have also constructed the co-expression network between lncRNA and mRNA, and established the circRNA-miRNA-mRNA in CAC based on this data. This study provides valuable insights to understand the RNA involvement and regulation in COPD, assisting future COPD investigations.
|
| |
|
| Overall design |
Examination of mRNAs, lncRNAs, miRNAs and circRNAs in peripheral blood of COPD patients
|
| |
|
| Contributor(s) |
Zhao D, Wang Y |
| Citation(s) |
35690768 |
| |
| Submission date |
Mar 16, 2022 |
| Last update date |
Jun 16, 2022 |
| Contact name |
Dahai Zhao |
| E-mail(s) |
[email protected]
|
| Organization name |
The Second Affiliated Hospital, Anhui Medical University
|
| Department |
Respiratory and Critical Care Medicine
|
| Street address |
Furong Road, 678
|
| City |
Hefei |
| State/province |
Anhui |
| ZIP/Postal code |
230601 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA816715 |
Less...
More...