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| Status |
Public on Jan 20, 2023 |
| Title |
in situ HiPore-C reveals higher-order 3D genome folding principles |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Eukaryotic genomes are folded into three-dimensional (3D) hierarchical architectures consisting of multi-way chromatin contacts that are involved in complex gene regulation. High-throughput chromosome conformation capture (Hi-C), chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) , and methods similar to these two rely on pairwise ligation of fragments in proximity in a population of cells, thus fail to reveal multi-way chromatin interactions in single allele at single fragment resolution. To explore higher-order chromatin interaction genome-widely, a straightforward strategy is integrating multi-fragment ligates preparation with third-generation sequencing technology. To achieve this purpose, we developed a protocol of in situ high throughput multi-way contact long read Pore-C sequencing (in situ HiPore-C)
We performed genome-wide multi-way contact profiling analysis using data obtained from in situ HiPore-C sequencing of GM12878 and K562 cell lines.
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| Overall design |
in situ HiPore-C sequencing for GM12878 and K562 cell lines.
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| Web link |
https://doi.org/10.1038/s41467-023-36899-x
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| |
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| Contributor(s) |
Xiao C, Hou C |
| Citation(s) |
36878904 |
| |
| Submission date |
May 09, 2022 |
| Last update date |
Apr 01, 2023 |
| Contact name |
Jiayong Zhong |
| Organization name |
Zhongshan Ophthalmic Centre, Sun Yat-sen University
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| Department |
State Key Laboratory of Ophthalmology, Clinical Research Center for Ocular Disease
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| Street address |
Jinsui Road No.7
|
| City |
Guangzhou |
| ZIP/Postal code |
510632 |
| Country |
China |
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| Platforms (1) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA836538 |
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