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| Status |
Public on Mar 20, 2010 |
| Title |
Activated Notch-induced transcriptional profile modulation in human primary dermal lymphatic endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Human Notch1 intracellular domain (NICD) was overexpressed in human primary lymphatic endothelial cells (LECs) for 10 and 24 hours by adenovirus. A GFP-control adenovirus-infected cells (24hours) and uninfected cells were also analysed as controls.
Total RNAs were harvested and subjected to Affymetrix U133A microarray.
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| Overall design |
Human primary lymphatic endothelial cells (LECs) were isolated from human foreskin and cultured and expanded to population passages 5~6. Healthy subconfluent primary LECs were infected with adenovirus expressing human Notch1 intracellular domain (NICD) for 10 or 24 hours. In parallel, LECs were also infected with a GFP-expressing control adenovirus for 24 hours. Uninfected LECs were also used as a negative control in the same experiments
*** CEL files unavailable/unrecoverable. ***
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| Contributor(s) |
Hong YK |
| Citation(s) |
20351309, 22719258 |
| |
| Submission date |
Mar 19, 2010 |
| Last update date |
Aug 27, 2019 |
| Contact name |
Young Kwon Hong |
| E-mail(s) |
[email protected]
|
| Organization name |
Norris Cancer Center
|
| Street address |
1441 Eastlake Ave NOR6336
|
| City |
los angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA124481 |
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