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Series GSE212625 Query DataSets for GSE212625
Status Public on Dec 12, 2022
Title PALI1 Promotes Tumor Growth through Competitive Recruitment of PRC2 to G9A-target Chromatin for Dual Epigenetic Silencing [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary PALI1 is a newly identified accessory protein of the Polycomb Repressive Complex 2 (PRC2) that catalyzes H3K27 methylation. However, the roles of PALI1 in cancer are yet to be defined. Here we report that PALI1 is upregulated in advanced prostate cancer (PCa) and competes with JARID2 for binding to PRC2 core subunit SUZ12. PALI1 further interacts with the H3K9 methyltransferase G9A, bridging the formation of a unique G9A-PALI1-PRC2 super-complex that occupies a subset of G9A-target genes to mediate dual H3K9/K27 methylation and gene repression. Many of these genes are developmental regulators induced upon cell differentiation, and their loss in PCa predicts poor prognosis. Accordingly, PALI1 and G9A drive PCa cell proliferation and invasion in vitro and xenograft tumor growth in vivo. Collectively, our study shows that PALI1 harnesses two central epigenetic mechanisms to suppress cellular differentiation and promote tumorigenesis, which can be targeted by dual EZH2 and G9A inhibition.
 
Overall design Examination of H3K27me3 and H3K9me2 modification along with SUZ12, PALI and G9a bound chromatin loci in human prostate cancer cells. There are 27 ChIP-Seq samples with pLKO/siCtrl as the control/reference.
 
Contributor(s) Yu J, Fong KW
Citation(s) 36476474
Submission date Sep 02, 2022
Last update date Dec 14, 2022
Contact name Jindan Yu
E-mail(s) [email protected]
Organization name Emory University
Department Urology
Lab Jindan Yu's lab
Street address E330, 1760 Haygood Dr NE
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platforms (1)
GPL15456 Illumina HiScanSQ (Homo sapiens)
Samples (27)
GSM6541881 CUT&RUN_igG
GSM6541882 pLKO_input
GSM6541883 siCtrl_G9a ChIP-seq_Rep1
This SubSeries is part of SuperSeries:
GSE175697 PALI1 Promotes Tumor Growth through Competitive Recruitment of PRC2 to G9A-target Chromatin for Dual Epigenetic Silencing
Relations
BioProject PRJNA876279

Download family Format
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Supplementary file Size Download File type/resource
GSE212625_RAW.tar 2.4 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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