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| Status |
Public on Sep 16, 2022 |
| Title |
Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling (Mouse I). |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pulmonary fibrosis results from dysregulated repair of damaged tissue caused by persistent injury of lung epithelium. Multiple cell types in the lung are involved in the process of repair. During lung fibrogenesis, normal endothelial cells (EC) are re-programmed into fibrosis-associated EC. Transcriptional factors that control re-programming are poorly understood. Using single cell RNA-sequencing of EC from donor and idiopathic pulmonary fibrosis (IPF) lungs, and lungs from bleomycin-treated mice, we identified endothelial transcription factors (TF) that were differentially expressed during fibrosis. Focusing on one of endothelial TF, FOXF1, we demonstrated that FOXF1 is decreased in EC within human IPF and mouse bleomycin-injured fibrotic lungs.
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| Overall design |
Using the 10x Chromium platform sequencing, transcriptomes of single cells from normal mouse lung and bleomycin treated mouse lung were analyzed.
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| Contributor(s) |
Bian F, Le T, Zhao S, Bacchetta M, Hozain AE, Tipograf Y, Xu Y, Kalin TV |
| Citation(s) |
29642003, 37137915 |
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| Submission date |
Sep 09, 2022 |
| Last update date |
Aug 02, 2023 |
| Contact name |
Yan Xu |
| E-mail(s) |
[email protected]
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| Phone |
513-6368921
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| Organization name |
Cincinnati Children's Hospital Medical Center
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| Street address |
3333 Burnet Ave
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| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE213018 |
Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling |
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| Relations |
| BioProject |
PRJNA878788 |
