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| Status |
Public on Feb 29, 2024 |
| Title |
Generation of induced pluripotent stem (iPS) cells using non-viral magnet-based nanofection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Using magneto-nanofection, iPS cells were efficiently generated by the transient expression of iPS genes in MEF (mouse embryonic fibroblast), suggesting that the non-viral magneto-nanofection method can be used for the efficient generation of iPS cells.
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| Overall design |
Plasmid DNAs containing the iPS genes oct4, sox2, klf4 and C-myc were introduced into MEF cells using magneto-nanofection. ESC media were changed daily for two days after transfection, and we could observe some colonies showing ES-like morphology 6-7 days after transfection. The colonies were picked up 12 days after transfection, and then each colony was separately transferred to a new dish containing STO feeder cells.
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| Contributor(s) |
Cho SG, Hwang D, Kim KP |
| Citation missing |
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| Submission date |
Apr 15, 2010 |
| Last update date |
Feb 29, 2024 |
| Contact name |
Sojeong Yun |
| E-mail(s) |
[email protected]
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| Organization name |
POSTECH
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| Department |
IBIO
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| Street address |
Hyoja-dong,Nam-gu
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| City |
Pohang, Kyungbuk |
| ZIP/Postal code |
790-784 |
| Country |
South Korea |
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| Platforms (1) |
| GPL6481 |
Illumina mouse-6 v1.1 expression beadchip [Array_Address_Id version] |
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| Samples (10)
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| GSM533658 |
E1 (KU-iN3), biological replicate 1 |
| GSM533659 |
E1 (KU-iN3), biological replicate 2 |
| GSM533660 |
E1 (KU-iN3), biological replicate 3 |
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| Relations |
| BioProject |
PRJNA126115 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21357_non-normalized.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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