 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 29, 2023 |
| Title |
Single-cell transcriptomics reveals glial cells integrate homeostatic and circadian processes to drive sleep-wake cycle |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The sleep-wake cycle is determined by a circadian and a sleep homeostatic process. However, the molecular impact of these two processes and their interaction on different cell populations in the brain remain unknown. To fill this gap, we have profiled the single-cell transcriptome of adult fruit fly brains across the sleep-wake cycle and different circadian times. We show cell type-specific transcriptomic changes between sleep/wakefulness states, different levels of sleep drive, and varying circadian times, with glial cells displaying the largest variations. Furthermore, the cell types whose transcriptomic dynamics correlate with the sleep homeostat or circadian clock are largely non-overlapping, with the exception of glial cells. Diminishing the circadian clock only in glial cells impairs the homeostatic sleep rebound after sleep deprivation. These findings reveal a comprehensive picture of different effects of sleep homeostatic and circadian processes on different cell types and define glial cells as the interaction sites of these two processes to determine sleep-wake dynamics.
|
| |
|
| Overall design |
Single-cell RNA-seq (10X Genomics) on adult Drosophila brain at different sleep, wake, sleep deprivation and Zeitgeber (ZT) times
|
| |
|
| Contributor(s) |
Dopp J, Ortega A, Davie K, Poovathingal S, Baz E, Liu S |
| Citation(s) |
38263460 |
| |
| Submission date |
Dec 18, 2022 |
| Last update date |
Feb 15, 2024 |
| Contact name |
Kristofer James Davie |
| Organization name |
Vlaams Instituut voor Biotechnologie
|
| Department |
Center for Brain and Disease Research
|
| Street address |
Herestraat 49
|
| City |
Leuven |
| ZIP/Postal code |
3000 |
| Country |
Belgium |
| |
|
| Platforms (2) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
|
| Samples (7)
|
| GSM6856710 |
DGRP_Mix_Sleep_Wakefulness_Run1, replicate 1, scRNA-seq |
| GSM6856711 |
DGRP_Mix_Sleep_Wakefulness_Run2, replicate 1, scRNA-seq |
| GSM6856712 |
DGRP_Mix_Sleep_Wakefulness_Run3, replicate 1, scRNA-seq |
| GSM6856713 |
DGRP_Mix_Sleep_Wakefulness_Run4, replicate 1, scRNA-seq |
| GSM6856714 |
DGRP_Mix_Sleep_Wakefulness_Run5, replicate 1, scRNA-seq |
| GSM6856715 |
DGRP_Mix_Sleep_Wakefulness_Run6, replicate 1, scRNA-seq |
| GSM6856716 |
DGRP_Mix_Sleep_Wakefulness_Run7, replicate 1, scRNA-seq |
|
| Relations |
| BioProject |
PRJNA913579 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE221239_All_Runs_PerLine_Metadata.csv.gz |
506 b |
(ftp)(http) |
CSV |
| GSE221239_RAW.tar |
1.1 Gb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...