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| Status |
Public on Jan 05, 2024 |
| Title |
Transcriptome analysis in transgenerational epigenetic inheritance |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
The study was performed towards understanding transgenerational epigenetic inheritance at transcriptome level. The founder males of F0 generation (actin-GAL4/Pin; UASRNAi-mts/+), and the resulting, male-line derived F1 (Pin/+; +/+), and F2 (+/+; +/+) generation individuals were used as test lineage, and the wild-type w1118 progeny as control lineage flies. The fly strains used for generating founder F0 line included Pin/CyO-dYFP; UASRNAi-mts/MKRS, actin-GAL4/CyO; +/+, and w1118. Precursor strains, as appropriate, were outcrossed with w1118 to minimize genetic background differences.
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| Overall design |
F0 males (actin-GAL4/Pin; UASRNAi-mts) were mated with w1118 females to obtain F1 progeny (Pin/+; +/+), and F1 males mated with w1118 females to obtain F2 progeny (+/+; +/+) in the test lineage. In parallel, w1118 flies were self-crossed, and the control lineage flies obtained. Three to five biological replicates were used.
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| Contributor(s) |
Pinreddy K, Sharma A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 10, 2023 |
| Last update date |
Jan 05, 2024 |
| Contact name |
Abhay Sharma |
| E-mail(s) |
[email protected]
|
| Phone |
9212036005
|
| Organization name |
CSIR Institute of genomics and integrative biology
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| Department |
Molecular medicine
|
| Street address |
Sukhdev vihar
|
| City |
New Delhi |
| State/province |
New Delhi |
| ZIP/Postal code |
110025 |
| Country |
India |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA922492 |
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