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| Status |
Public on Aug 05, 2010 |
| Title |
L2_body_wall_muscle_tiling_array |
| Project |
modENCODE
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| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
modENCODE_submission_465
This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Keywords: Transcript tiling array analysis
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| Overall design |
EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: SD1075 (engineered, target gene myo-3 tagged by 3xFlag::PAB-1); Tissue: body wall muscle; Developmental Stage: larva mid-L2 20dC 22h post-L1; Genotype: gaIs146 [(myo-3p::FLAG::PAB-1) + (sur-5::GFP)]; Sex: mixed Male and Hermaphrodite population; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Antibody EZview Red ANTI-FLAG® M2 Affinity Gel (target is FLAG); Strain SD1075 (engineered, target gene myo-3 tagged by 3xFlag::PAB-1); temperature 20; Developmental Stage larva mid-L2 20dC 22h post-L1; Tissue body wall muscle
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| Web link |
http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
http://www.modencode.org
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| Contributor(s) |
Waterston B, Miller D, Gerstein M, Wilson M, Argiwal A, Reinke V |
| Citation(s) |
21177967 |
| BioProject |
PRJNA168961 |
| |
| Submission date |
Jul 28, 2010 |
| Last update date |
May 02, 2013 |
| Contact name |
DCC modENCODE |
| E-mail(s) |
[email protected]
|
| Phone |
416-673-8579
|
| Organization name |
Ontario Institute for Cancer Research
|
| Lab |
modENCODE DCC
|
| Street address |
MaRS Centre, South Tower, 101 College Street, Suite 800
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| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL5634 |
[Ce25b_MR] Affymetrix C. elegans Tiling 1.0R Array |
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| Samples (3) |
| GSM571446 |
L2 body wall muscle tiling array extraction1_array1 |
| GSM571447 |
L2 body wall muscle tiling array extraction2_array1 |
| GSM571448 |
L2 body wall muscle tiling array extraction3_array1 |
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