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| Status |
Public on Jun 14, 2024 |
| Title |
Effect of overexpress of lncFABP4 on gene expression during adipogenic differentiation of buffalo intramuscular adipocytes |
| Organism |
Bubalus bubalis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the function lncFABP4 in the regulation of adipogenic differentiation, we overexpressed lncFABP4 in buffalo intramuscular adipocytes
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| Overall design |
First, intramuscular adipocytes were planted in six-well plates in triplicate. When the cells reach 80% confluence, transduction was performed. Adenovirus were added to cells at the indicated MOI. Then, The culture medium was exchanged three hours later. Next, When transduction was performed for 48 hours, cells were treated with an inducing medium. Finally, Cells were collected for RNA sequencing after 48 hours of induction.
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| Contributor(s) |
Huang J |
| Citation(s) |
38703069 |
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| Submission date |
Jun 14, 2023 |
| Last update date |
Sep 15, 2024 |
| Contact name |
Huang JiePing |
| E-mail(s) |
[email protected]
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| Organization name |
Guangxi University
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| Street address |
Xixiangtang District
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| City |
Nanning |
| ZIP/Postal code |
530000 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| GSM7476430 |
Intramuscular adipocytes, Ad_EGFP transduction, Day4, rep1 [Ad_EGFP-1] |
| GSM7476431 |
Intramuscular adipocytes, Ad_EGFP transduction, Day4, rep2 [Ad_EGFP-2] |
| GSM7476432 |
Intramuscular adipocytes, Ad_EGFP transduction, Day4, rep3 [Ad_EGFP-3] |
| GSM7476433 |
Intramuscular adipocytes, Ad_ lncFABP4 transduction, Day4, rep1 [Ad_ lncFABP4-1] |
| GSM7476434 |
Intramuscular adipocytes, Ad_ lncFABP4 transduction, Day4, rep2 [Ad_ lncFABP4-2] |
| GSM7476435 |
Intramuscular adipocytes, Ad_ lncFABP4 transduction, Day4, rep3 [Ad_ lncFABP4-3] |
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| Relations |
| BioProject |
PRJNA983733 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE234915_Processed_data_identifed_transcripts.xlsx |
1.7 Mb |
(ftp)(http) |
XLSX |
| GSE234915_Processed_data_sequence_for_identifed_transcripts.fa.gz |
16.4 Mb |
(ftp)(http) |
FA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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