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| Status |
Public on Aug 20, 2010 |
| Title |
Gene expression study in choroid plexus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The choroid plexus (CP) constitutes one of the blood–brain barriers – the blood cerebrospinal fluid barrier. It is formed by a monolayer of epithelial cells located within the brain ventricles and in addition to be responsible for the formation of the cerebrospinal fluid (CSF) is ideally positioned to transmit signals into and out of the brain. Specifically, here, we evaluated the overall kinetic response of the mouse CP to peripheral administration of lipopolysaccharide (LPS) that is the Gram-negative bacteria cell wall. This study shows that as soon as 1h after the injection, the CP responds by altering the expression of several genes. This response reaches a maximum at 3h and returns to the basal profile for most of the transcripts at 72h. To do this analysis we compared the CP transcriptome of LPS injected animals with the CP transcriptome of saline injected animals and using this we also dissect the CP basal transcriptome. From this analysis we confirm that the genes most highly expressed were those implicated in energy metabolism (oxidative phosphorylation, glycolysis/gluconeogenesis) and in ribosomal function, which is in agreement with the secretory nature of the CP. Finally, we also describe genes not previously identified as being expressed in the basal CP.
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| Overall design |
Gene expression study in choroid plexus. Time series after acute LPS treatment
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| Contributor(s) |
Marques F, Sousa JC, Coppola G, Gao F, Puga R, Brentani H, Geschwind D, Sousa N, Correia-Neves M, Palha JA |
| Citation(s) |
21349147 |
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| Submission date |
Aug 19, 2010 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Giovanni Coppola |
| E-mail(s) |
[email protected]
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| Phone |
310-794-4172
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| Organization name |
UCLA
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| Department |
Psychiatry and Neurology
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| Lab |
Neurogenetics
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| Street address |
1524 Gonda, 695 Charles Young Drive South
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL6100 |
Illumina mouseRef-8 v1.0 expression beadchip |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA130879 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23714_RAW.tar |
2.6 Mb |
(http)(custom) |
TAR |
| GSE23714_non-normalized.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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