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| Status |
Public on Oct 31, 2023 |
| Title |
A Diversity of Novel Type-2 Innate Lymphoid Cell Subpopulations Revealed During Tumour Expansion |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Type 2 innate lymphoid cells (ILC2s) perform vital functions in orchestrating humoral immune responses, facilitating tissue remodelling, and ensuring tissue homeostasis. Additionally, in a role that has garnered considerably less attention, ILC2s can also enhance Th1-related cytolytic T lymphocyte immune responses against tumours. Studies have thus far generally failed to address the mystery of how one ILC2 cell-type can participate in a multiplicity of functions. Here we utilized single cell RNA sequencing analysis to create the first comprehensive atlas of naïve and tumour-associated lung ILC2s and discover multiple unique subtypes of ILC2s equipped with developmental gene programs that become skewed during tumour expansion favouring inflammation, antigen processing, immunological memory and Th1-related anti-tumour CTL responses. The discovery of these new subtypes of ILC2s challenges current paradigms of ILC2 biology and provides an explanation for their diversity of function.
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| Overall design |
Primary ILC2s isolated, purified, enriched and single-cell sequenced at different stages of tumour growth in WT C57BL/6 mice to investigate the immune surveillance dynamics during tumour progression through gene expression changes. Samples include tumour-carrying activated, tumour-carrying non-activated and naive non-activated as control.
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| Contributor(s) |
Xia CW, Saranchova I, de Lucía Finkel P, Besoiu S, Munro L, Pfeifer CG, Haegert A, Lin Y, Le Bihan S, Collins C, Jefferies WA |
| Citation(s) |
38172434 |
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| Submission date |
Oct 30, 2023 |
| Last update date |
Jan 16, 2024 |
| Contact name |
Wilf Jefferies |
| E-mail(s) |
[email protected]
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| Organization name |
UBC
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| Street address |
#301-2185 East Mall
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| City |
Vancouver |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA1033525 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE246502_RAW.tar |
154.0 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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