 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 22, 2024 |
| Title |
Gene expression data from precision cut tumor slices from head and neck or mesothelioma tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
As cancer immunotherapy and precision medicine dynamically evolve, there is greater need for pre-clinical models that can better replicate the intact tumor and its complex tumor microenvironment (TME). Precision-cut tumor slices (PCTS) have recently emerged as an ex vivo human tumor model, offering the opportunity to study individual patient responses to targeted therapies, including immunotherapies. However, little is known about the physiologic status of PCTS and how they can be utilized in view of the intra-tumoral heterogeneity. In this study, we generated PCTS from head and neck cancers (HNC) and mesotheliomas (Meso) and studied their viability in culture, the extent of variability in serial PCTS, and whether tumor immune response can be measured following PCTS T cell activation. We also undertook transcriptomic analyses to understand the changes that occur in the timeframe between PCTS generation and up to 72hr in culture. Our findings suggest that PCTS viability can be tumor-specific and culture conditions-dependent, but an overall viability of up to 72 hrs was observed. Tumor heterogeneity is present and can skew findings if appropriate experimental design and multiple control PCTS are not considered. Activation of endogenous T cells and measurement of immune responses is possible, however, our transcriptomic analyses showed major changes occurring during the first 24hr culture period of PCTS, involving genes related to wound healing, extracellular matrix, hypoxia, and IFNγ-dependent pathways. Our data suggest the PCTS model may be especially useful for studies involving exogenous immune therapies, such as adoptive cell therapy, rather than for the study of the effects of therapy on endogenous immune systems.
|
| |
|
| Overall design |
Tumors were collcted from patients with head and neck or mesothelion and sectioned into thin slices. RNA was collected and sequencing performed on slices fresh after sectioning or after 24, 48 or 72 hours in culture.
|
| |
|
| Contributor(s) |
Sumita T, Caitlin T, Maria L, Joshua B, Robert B, Karthik R, Sunil S, Steven A, Astero K |
| Citation(s) |
38744944 |
| |
| Submission date |
Dec 12, 2023 |
| Last update date |
May 22, 2024 |
| Contact name |
Caitlin Tilsed |
| E-mail(s) |
[email protected]
|
| Phone |
2674213934
|
| Organization name |
The University of Pennsylvania
|
| Street address |
407F 4312 Chestnut St
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (26)
|
|
| Relations |
| BioProject |
PRJNA1051641 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE250038_fresh_24hr.xlsx |
2.0 Mb |
(ftp)(http) |
XLSX |
| GSE250038_timecourse.xlsx |
3.6 Mb |
(ftp)(http) |
XLSX |
| Raw data not provided for this record |
|
|
|
|
 |
Less...
More...