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| Status |
Public on Nov 13, 2024 |
| Title |
Intrinsically Disordered Regions Steer the Function Coordination of the Traveling Chromatin Modifier During Pol II Elongation (ChIP-Seq) |
| Organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The Rpd3S histone deacetylase complex suppresses cryptic initiation and maintains genomic integrity by deacetylating transcribed nucleosomes subsequent to RNA Pol II passage. Recent cryo-EM structure analysis of Rpd3S emphasized the significance of two asymmetrical Rco1/Eaf3 dimers in nucleosome binding. However, the mechanism by which Rpd3S interacts with nucleosomal substrates while traveling with elongating Pol II remains elusive. In this study, we elucidate the crucial role of the Rco1 N-terminal Intrinsically Disordered Region (IDR) in regulating Pol II association. Mutations within the K/R cluster of Rco1 IDR significantly impede Rpd3S binding to CTD, while leaving the overall complex integrity and nucleosome recognition unaffected. Moreover, we identify the Rco1-PHD1 and Eaf3-CHD domains as the minimal module responsible for specific interaction with Ser5-phosphorylated CTD. The Rco1 IDR functions by relieving autoinhibition from its C-terminal regions, enabling the binding of PHD-CHD to CTD. Additionally, we uncover an evolutionarily conserved mechanism governing the distinct roles of the asymmetrical Rco1/Eaf3 dimers, which coordinate nucleosome engagement and Pol II binding. These findings offer valuable new perspectives on the functional dynamics of mobile epigenetic modifying complexes that accompany processive machinery.
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| Overall design |
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for H4 acetylation in wild-type and 13K/R>A mutant yeast strains.
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| Contributor(s) |
Pan Y, Liu M, Zhang Q, Li B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 30, 2024 |
| Last update date |
Nov 14, 2024 |
| Contact name |
Bing Li |
| E-mail(s) |
[email protected]
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| Organization name |
Shanghai Jiao Tong University School of medicine
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| Department |
Department of Biochemistry and Molecular Cell Biology
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| Street address |
South Chongqing Road 280
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| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (2) |
| GPL27812 |
Illumina NovaSeq 6000 (Saccharomyces cerevisiae) |
| GPL28961 |
Illumina NovaSeq 6000 (Schizosaccharomyces pombe) |
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| Samples (18)
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| GSM8046389 |
rco1-delta, H4Ac, ChIP, rep2 |
| GSM8046390 |
Rco1-13KR-to-A, H4Ac, ChIP, rep1 |
| GSM8046391 |
Rco1-13KR-to-A, H4Ac, ChIP, rep2 |
| GSM8046392 |
Rco1-13KR-to-A_CO, H4Ac, ChIP, rep1 |
| GSM8046393 |
Rco1-13KR-to-A_CO, H4Ac, ChIP, rep2 |
| GSM8046394 |
wild-type, input |
| GSM8046395 |
Rco1-13KR-to-A_CO, input |
| GSM8046396 |
wild-type, H4Ac, ChIP,rep1 |
| GSM8046397 |
wild-type, H4Ac, ChIP,rep2 |
| GSM8046398 |
cph1-delta,H4Ac, ChIP,rep1 |
| GSM8046399 |
cph1-delta,H4Ac, ChIP,rep2 |
| GSM8046400 |
cph2-delta,H4Ac, ChIP,rep1 |
| GSM8046401 |
cph2-delta,H4Ac, ChIP,rep2 |
| GSM8046402 |
wild-type, input,rep1 |
| GSM8046403 |
wild-type, input,rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE254580 |
Intrinsically Disordered Regions Steer the Function Coordination of the Traveling Chromatin Modifier During Pol II Elongation |
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| Relations |
| BioProject |
PRJNA1071290 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE254578_RAW.tar |
26.4 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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