 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 16, 2024 |
| Title |
Lineage-specific genomic imprinting in the ZNF791 locus [BiSulfite-seq] |
| Organism |
Sus scrofa |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
To identify genomic imprinting in pigs, we generated parthenogenetic porcine embryos alongside biparental normal embryos, and then performed whole-genome bisulfite sequencing and RNA sequencing on these samples. In our analyses, we discovered a maternally methylated differentially methylated region within the orthologous ZNF791 locus in pigs, while identifying both a major imprinted isoform of the ZNF791-like gene and an imprinted antisense transcript that has not been previously annotated. Importantly, our comparative analyses of the orthologous ZNF791 gene in various eutherian mammals, including humans, non-human primates, rodents, artiodactyls, and dogs, revealed that this gene is subjected to genomic imprinting exclusively in domesticated animals, thereby highlighting lineage-specific imprinting.
|
| |
|
| Overall design |
Whole genome bisulfite sequencing was performed on parthenogenetically activated embryos and control pig embryos.
|
| |
|
| Contributor(s) |
Ahn J, Hwang S, Lee K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 08, 2024 |
| Last update date |
Oct 17, 2024 |
| Contact name |
Jinsoo Ahn |
| E-mail(s) |
[email protected]
|
| Organization name |
Ohio State Univesity
|
| Street address |
2029 Fyffe Rd
|
| City |
Columbus |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA1097645 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE263495_RAW.tar |
84.1 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...