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| Status |
Public on Jan 11, 2011 |
| Title |
Phenotype, Function and Gene Expression Profiles of PD-1 high CD8 T cells in Healthy Human Adults |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
T cell dysfunction is an important feature of many chronic viral infections. In particular, it was shown that PD-1 regulates T cell dysfunction during chronic LCMV infection in mice and PD-1 high cells exhibit an intense exhausted gene signature. These findings were extended to human chronic infections such as HIV, HCV and HBV. However, it is not known if PD-1 high cells of healthy humans have the traits of exhausted cells. In this study, we provide a comprehensive description of phenotype, function and gene expression profiles of PD-1 high versus PD-1 low CD8 T cells in the peripheral blood of healthy human adults as following: 1) The percentage of naive and memory CD8 T cells varied widely in the peripheral blood cells of healthy humans and PD-1 was expressed by the memory CD8 T cells. 2) PD-1 high CD8 T cells in healthy humans did not significantly correlated with the PD-1 high exhausted gene signature of HIV specific human CD8 T cells or chronic LCMV specific CD8 T cells from mice. 3) PD-1 expression did not directly affect the ability of CD8 T cells to secrete cytokines in healthy adults. 4) PD-1 was expressed by the effector memory (TEM) compared to ‘terminally differentiated effector’ (TEMRA) CD8 T cells. 5) Finally, an interesting inverse relationship between CD45RA and PD-1 expression was observed.
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| Overall design |
We used highly purified PD-1 high and PD-1 low from six healthy adult individuals and naive CD8 T cell populations from four of those individuals for gene expression studies
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| Contributor(s) |
Duraiswamy J, Ahmed R |
| Citation(s) |
21383243 |
| |
| Submission date |
Jan 07, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Jaikumar Duraiswamy |
| E-mail(s) |
[email protected]
|
| Organization name |
Emory Univesity
|
| Department |
Emory Vaccine Center and Department of Microbiology & Immunology
|
| Lab |
Ahmed lab
|
| Street address |
1510, Clifton Road, G211, Rollins Research Center
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA136499 |
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