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Series GSE27834 Query DataSets for GSE27834
Status Public on Jun 01, 2011
Title miR-371-3 expression predicts neural differentiation potential in human pluripotent stem cells
Organism Homo sapiens
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary The use of pluripotent stem cells in regenerative medicine and disease modeling is complicated by the variation in differentiation properties between lines. In this study, we characterized 13 human embryonic stem cell. (hESC) and 26 human induced pluripotent stem cell (hiPSC) lines to identify markers that predict neural differentiation behavior. At a general level, markers previously known to distinguish mouse ESCs from epiblast stem cells (EpiSCs) correlated with neural differentiation behavior. More specifically, quantitative analysis of miR-371-3 expression prospectively identified hESC and hiPSC lines with differential neurogenic differentiation propensity and in vivo dopamine neuron engraftment potential. Transient KLF4 transduction increased miR-371-3 expression and altered neurogenic behavior and pluripotency marker expression. Conversely, suppression of miR- 371-3 expression in KLF4-transduced cells rescued neural differentiation propensity. miR-371-3 expression level therefore appears to have both a predictive and a functional role in determining human pluripotent stem cell neurogenic differentiation behavior.
 
Overall design [mRNA profiling (Illumina)]: Four human ESC lines (H9, I4, I6, HUES6) at undifferentiation stages were purified with stem cell surface marker SSEA4 and subjected to RNA extraction and hybridization on Illumina microarrays. Each sample has 3 biological repeats, one of which has two technical repeats.

[miRNA profiling (Agilent)]: Four human ESC lines (H9, I4, I6, HUES6) at undifferentiation stages were purified with stem cell surface marker SSEA4 and subjected to RNA extraction and hybridization on Agilent microarrays. Each sample has 3 biological repeats, one of which has two technical repeats.
 
Contributor(s) Kim H, Studer L
Citation(s) 21624813
Submission date Mar 08, 2011
Last update date Jan 18, 2013
Contact name Jeffrey Zhao
Organization name Memorial Sloan Kettering Cancer Center
Department Genomics Core
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (2)
GPL6104 Illumina humanRef-8 v2.0 expression beadchip
GPL6955 Agilent-016436 Human miRNA Microarray 1.0 (Feature Number version)
Samples (32)
GSM687186 H9 hESCs biological rep 1-1 [mRNA]
GSM687187 HUES6 hESCs biological rep 1-1 [mRNA]
GSM687188 I4 hESCs biological rep 1-1 [mRNA]
Relations
BioProject PRJNA138025

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27834_Illumina_complete_normalized_data.txt.gz 3.2 Mb (ftp)(http) TXT
GSE27834_Illumina_non-normalized_data.txt.gz 4.1 Mb (ftp)(http) TXT
GSE27834_RAW.tar 27.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data are available on Series record
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