|
| Status |
Public on Jun 01, 2011 |
| Title |
Expression profiles of mouse glioma-initiating cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify factors involved in glioma-initiating cells (GICs), we compared gene expressions between GIC-like cells and non-GICs.
|
| |
|
| Overall design |
p53-deficient Neural stem cells (NSCs), oligodendrocyte precursor cells (OPCs), and astrocytes (ASTs) were transfected with pCMS-EGFP-HRasL61 and pBabe-neo by electroporation and cultured in 0.5 mg/ml neomycin. The GFP-positive stable NSCs (NSCL61s), OPCs (OPCL61s) and ASTs (ASTL61s) were purified by flow cytometry. Total RNA was prepared using a RNeasy Mini Kit (QIAGEN) and used for the expression analysis.
|
| |
|
| Contributor(s) |
Kondo T |
| Citation(s) |
21360625 |
| |
| Submission date |
Mar 22, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Toru Kondo |
| E-mail(s) |
[email protected]
|
| Phone |
+81-11-706-6082
|
| Organization name |
Hokkaido University
|
| Department |
Institute for Genetic Medicine
|
| Lab |
Division of Stem Cell Biology
|
| Street address |
Kita-15, Nishi-7, Kita-ku,
|
| City |
Sapporo |
| ZIP/Postal code |
060-0815 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA139747 |
Less...
More...