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| Status |
Public on Nov 06, 2024 |
| Title |
Decoupling the Biochemical and Mechanical Effects of Muscle Exercise on Motor Neurons with 2.5D Actuating Matrices [240614Ram_RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We wanted to query the differences and similarities between our wild-type C2C12 cell line and our optogenetic ChR2 C2C12 myoblasts on the same day of differentiation. After 6 days on muscle differentiation medium, we extracted the RNA and sequenced it for 6 wells each.
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| Overall design |
Transcriptomic comparison of wild-type C2C12 and ChR2-optogenetic C2C12
The O1-O6 samples were six wells of optogenetic C2C12 myoblast that were differentiated in high glucose medium with IGF and aminocaproic acid for six days prior to RNA extraction. Samples WT1-WT6 are the control samples which underwent the same culture conditions but using a WT line.
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| Contributor(s) |
Angel B, Ritu R, Butty V |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 06, 2024 |
| Last update date |
Nov 06, 2024 |
| Contact name |
Vincent Butty |
| E-mail(s) |
[email protected]
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| Organization name |
Massachusetts Institute of Technology
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| Department |
Biology / Koch Institute / Bioengineering / CEHS
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| Lab |
BioMicro Center / IGE
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| Street address |
77 Massachusetts Avenue Bldg 68-317A
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE281242 |
Decoupling the Biochemical and Mechanical Effects of Muscle Exercise on Motor Neurons with 2.5D Actuating Matrices |
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| Relations |
| BioProject |
PRJNA1182793 |
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