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| Status |
Public on Sep 14, 2011 |
| Title |
Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer [Expression Array] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We report the dual role of FoxA1 in androgen receptor recruitment to the chromatin of androgen responsive prostate cancer cell line LNCaP-1F5 using ChIP-sequencing. Depletion of FoxA1 reprograms both androgen and glucocorticoid receptor recruitment and subsequent gene expression. The ChIP-seq has been performed using AR, FoxA1, GR, H3K4me2 antibodies. We have also mapped the DNaseI-hypersensitive sites (DHS) using deep sequencing.
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| Overall design |
LNCaP-1F5 cells were depleted of FoxA1 using siRNAs. Parental cells and FoxA1 depleted cells (siFoxA1) were treated with vehicle or 100 nM DHT or (100 nM DEX) for 24 hours followed by RNA isolation and hybridization to Illumina arrays. All the experiments have been performed in biological duplicates. Parental cells treated with DHT (or DEX) were analyzed for differentially expressed genes compared to vehicle treated parental cells. Similarly siFoxA1 cells treated with DHT (or DEX) were analyzed for differentially expressed genes compared to vehicle treated siFoxA1 cells.
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| Contributor(s) |
Sahu B, Laakso M, Ovaska K, Mirtti T, Lundin J, Rannikko A, Sankila A, Turunen J, Lundin M, Konsti J, Vesterinen T, Nordling S, Kallioniemi O, Hautaniemi S, Jänne OA |
| Citation(s) |
21915096 |
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| Submission date |
Jul 12, 2011 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Olli A. Jänne |
| E-mail(s) |
[email protected]
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| Phone |
+358919125040
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| Organization name |
University of Helsinki
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| Department |
Inst. of Biomedicine/Physiology
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| Lab |
Androgen Receptor Laboratory
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| Street address |
Haartmaninkatu-8
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| City |
Helsinki |
| ZIP/Postal code |
FI-00014 |
| Country |
Finland |
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| Platforms (2) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE30624 |
Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer |
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| Relations |
| BioProject |
PRJNA154783 |
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