|
| Status |
Public on Aug 18, 2011 |
| Title |
Global gene expression analysis of three different types of mouse motor neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of gene expression differences in three types of mouse motor neurons: (1) those harvested from E13.5 mouse embryos; (2) those derived from embryonic stem cells by directed differentiation; and (3) those made from mouse embryonic fibroblasts by transcription factor reprogramming.
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| Overall design |
Hb9::GFP+ mouse motor neurons were obtained by three methods [(1) dissection of Hb9::GFP-transgenic E13.5 mouse embryo spinal cord; (2) directed differentiation of an Hb9::GFP-transgenic mouse embryonic stem cell line, V6.5; (3) reprogramming of Hb9::GFP-transgenic mouse embryonic fibroblasts by overexpression of 10 transcription factors]. Each type of motor neuron was purified by FACS and harvested in Trizol. Total RNA was purified and prepared for hybridization onto Illumina MouseRef-8.
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| |
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| Contributor(s) |
Son EY, Ichida JK, Eggan K |
| Citation(s) |
21852222 |
| |
| Submission date |
Aug 02, 2011 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Esther Yesde Son |
| E-mail(s) |
[email protected]
|
| Phone |
617-496-5611
|
| Fax |
617-384-5611
|
| Organization name |
Harvard University
|
| Department |
Stem Cells and Regenerative Biology
|
| Lab |
Eggan
|
| Street address |
7 Divinity Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (10)
|
| GSM771424 |
Mouse embryonic motor neuron_GFP hi_rep1 |
| GSM771425 |
Mouse embryonic motor neuron_GFP med_rep1 |
| GSM771426 |
Mouse embryonic motor neuron_GFP hi_rep2 |
| GSM771427 |
Mouse embryonic motor neuron_GFP med_rep2 |
| GSM771428 |
Mouse ES-derived motor neuron_GFP hi_rep1 |
| GSM771429 |
Mouse ES-derived motor neuron_GFP med_rep1 |
| GSM771430 |
Mouse ES-derived motor neuron_GFP hi_rep2 |
| GSM771431 |
Mouse ES-derived motor neuron_GFP med_rep2 |
| GSM771432 |
Mouse induced motor neuron_GFP hi |
| GSM771433 |
Mouse induced motor neuron_GFP med |
|
| Relations |
| BioProject |
PRJNA144825 |
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