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Series GSE32050 Query DataSets for GSE32050
Status Public on Oct 30, 2011
Title 5-hydroxymethylcytosine-mediated epigenetic dynamics during neurodevelopment and aging [5hmC Capture and Seq]
Organisms Homo sapiens; Mus musculus; Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary DNA methylation dynamics influence brain function and are altered in neurological disorders. 5-hydroxymethylcytosine (5-hmC), a DNA base derived from 5-methylcytosine (5mC) accounts for ~40% of modified cytosine in brain, and has been implicated in DNA methylation-related plasticity. Here we map 5-hmC genome-wide across three ages in mouse hippocampus and cerebellum, allowing assessment of its stability and dynamic regulation during postnatal neurodevelopment through adulthood. We find developmentally programmed acquisition of 5-hmC in neuronal cells. Epigenomic localization of 5-hmC-regulated regions reveals stable and dynamically modified loci during neurodevelopment and aging. By profiling 5-hmC in human cerebellum we establish conserved genomic features of 5-hmC. Finally, we implicate 5-hmC in neurodevelopmental disease by finding that its levels are inversely correlated with methyl-CpG-binding protein 2 (Mecp2) dosage, a protein encoded by a gene in which mutations cause Rett Syndrome. These data point toward critical roles for 5-hmC-mediated epigenetic modification in neurodevelopment and diseases.
 
Overall design Here we map 5-hmC genome-wide across three ages in mouse hippocampus and cerebellum, allowing assessment of its stability and dynamic regulation during postnatal neurodevelopment through adulthood. Profiling of 5-hmC in human cerebellum we establish conserved genomic features of 5-hmC. Finally, we implicate 5-hmC in neurodevelopmental disease by profiling 5-hmC in mouse cerebellum lacking MeCP2, a protein encoded by a gene in which mutations cause Rett Syndrome.
 
Contributor(s) Szulwach KE, Jin P
Citation(s) 22037496
Submission date Sep 12, 2011
Last update date May 15, 2019
Contact name Keith E Szulwach
E-mail(s) [email protected]
Phone 404-712-0796
Organization name Emory University
Department Human Genetics
Lab Jin Lab
Street address 615 Michael Street, Whitehead Suite 301
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platforms (3)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL10287 Illumina Genome Analyzer II (Rattus norvegicus)
Samples (24)
GSM794654 5-hmC-mediated epigenetic dynamics (P7.Cb.1) [5hmC Capture and Seq]
GSM794655 5-hmC-mediated epigenetic dynamics (P7.Cb.2) [5hmC Capture and Seq]
GSM794656 5-hmC-mediated epigenetic dynamics (6wk.Cb.1) [5hmC Capture and Seq]
This SubSeries is part of SuperSeries:
GSE32188 5-hydroxymethylcytosine-mediated epigenetic dynamics during neurodevelopment and aging
Relations
SRA SRP008273
BioProject PRJNA155305

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