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| Status |
Public on Oct 22, 2011 |
| Title |
Identification of TDP-43 and FUS mRNA targets and pathways in the cytoplasmic compartment of motoneuronal NSC-34 cells |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
We performed RNA immunoprecipitation (IP) and microarray (RIP-chip) analyses to identify and compare the biological mRNA targets of two RNA-binding proteins (RBP), TDP-43 and FUS, associated to cytoplasmic ribonucleoprotein (RNP) complexes of motoneuronal NSC-34 cells with the final aim to unravel their role in mRNA transport, stability, and translation in neuronal cells.
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| Overall design |
To identify the transcripts contained and bound in the isolated RNP complexes, a chip analysis was performed using the recovered mRNAs from triplicate experimantal RIP samples (TDP-43-IP samples, the FUS-IP samples and the control IgG-IP sample).
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| Contributor(s) |
Ratti A, Colombrita C, Onesto E |
| Citation(s) |
22427648 |
| |
| Submission date |
Oct 21, 2011 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Claudia Colombrita |
| Organization name |
IRCCS Istituto Auxologico Italiano
|
| Street address |
Via Zucchi, 18
|
| City |
Cusano milanino |
| ZIP/Postal code |
20095 |
| Country |
Italy |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA149179 |
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