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| Status |
Public on Mar 23, 2012 |
| Title |
Time-course transcriptome measure of HoxB4-mediated HSC development from ES cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Efficient in vitro generation of hematopoietic stem cells (HSCs) from embryonic stem cells (ESCs) holds great promise for cell-based therapies of hematological diseases. To date, HoxB4 remains to be the most effective transcription factor (TF) whose over-expression in ESCs confers long-term repopulating ability to ESC-derived HSCs. Despite its importance, the components and dynamics of the HoxB4 transcriptional regulatory network is poorly understood, hindering efforts to develop a more efficient protocol for in vitro derivation of HSCs. Towards this goal, we performed global gene expression profiling and chromatin immunoprecipitation coupled with deep sequencing (ChIP-Seq) at four stages of the HoxB4-mediated HSC development. Joint analyses of ChIP-Seq and gene expression profiles unveil a number of global features of the HoxB4 regulatory network.
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| Overall design |
Cells from four time points of the developmental processes were collected, including days 0, 6, 16, and 26. Triplicate microarray hybridizations were done for cells from each time point.
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| Contributor(s) |
Tan K |
| Citation(s) |
22438249 |
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| Submission date |
Nov 25, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Kai Tan |
| Organization name |
University of California San Diego
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| Department |
Bioengineering
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE34014 |
Gene expression profiling and ChIP-Seq study of HoxB4-mediated HSC development from ES cells |
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| Relations |
| BioProject |
PRJNA156461 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33953_RAW.tar |
50.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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