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| Status |
Public on Dec 09, 2011 |
| Title |
Escherichia coli K-12 grown in the presence of glycerol exhibits upregulation in genes for acid stress chaperones and concomitant downregulation in genes involved in generation of acidic metabolites |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
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| Summary |
This study explores the effects of glycerol, on whole genome expression of Escherichia coli.
DNA microarray analysis imply that E. coli in the presence of glycerol generates acidic metabolites to which it adapts by upregulation of genes involved in acid stress and by simultaneously downregulating genes involved in high pH stress.
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| Overall design |
E.coli was grown in the presence and absence of glycerol. RNA was extracted from these samples, cDNA was prepared, fragmented cDNA was labled with labeling reagent and hybridized with Affymetrix E.coli2.0 genome array chips.Data was analysed to get the effect of glycerol on E.coli gene expression
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| Contributor(s) |
Shivaji S, Arunasri K, Adil M |
| Citation(s) |
24858919 |
| |
| Submission date |
Dec 08, 2011 |
| Last update date |
Aug 21, 2019 |
| Contact name |
kotakonda Arunasri |
| E-mail(s) |
[email protected]
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| Phone |
+919866459086
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| Organization name |
Centre for Cellular and Molecular biology
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| Street address |
Habsiguda
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| City |
Hyderabad |
| State/province |
Andhra Pradesh |
| ZIP/Postal code |
500007 |
| Country |
India |
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| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA149955 |
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