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| Status |
Public on Aug 03, 2012 |
| Title |
Identification of gene targets of Meis2 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The homeodomain protein Meis1 is essential for definitive hematopoiesis and vascular patterning in the mouse embryo. Meis2, another member of the same family, shares 82% protein identities with Meis1. Our present study suggested Meis2 exerts two distinguishable effects in differentiating ES cells. First, it increases the numbers of hematopoietic progenitors and extends their persistence in culture. Second, Meis2 skews hematopoietic differentiation by suppressing erythroid while enhancing megakaryocytic progenitor differentiation. To identify the underlying transcriptional bases of these actions, we carried out microarray analysis to compare the various populations of cells developing in ES differentiation cultures in the presence and absence of Meis2 induction.
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| Overall design |
ES cells with dox-inducible Meis2 (A2lox.Meis2) were differentiated as embryoid bodies (EBs) for 6 days before plating on OP9-GFP cell monolayers and cytokines, and treated with (+) or without (-) doxycycline (dox). Cells were purified by cell sorting on day 7 or 8 into various populations based on levels of CD41 expression: GFP-CD41-, GFP-CD41+ (day 7) and GFP-CD41-,GFP-CD41int, and GFP-CD41hi (day 8). Gene expression of these purified populations was determined by microarray analysis.
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| Contributor(s) |
Cai M, Murphy TL, Murphy KM |
| Citation(s) |
22665933 |
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| Submission date |
Dec 19, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Mi Cai |
| E-mail(s) |
[email protected]
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| Phone |
314-362-2004
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| Fax |
314-747-4888
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| Organization name |
Washington University School of Medicine
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| Department |
Immunology and Pathology
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| Lab |
Ken Murphy
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| Street address |
660 S. Euclid Ave.
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| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| GSM850829 |
CD41-, A2lox.Meis2 and OP9-GFP co-culture, D7, untreated |
| GSM850830 |
CD41+, A2lox.Meis2 and OP9-GFP co-culture, D7, untreated |
| GSM850831 |
CD41-, A2lox.Meis2 and OP9-GFP co-culture, D7, treated with dox |
| GSM850832 |
CD41+, A2lox.Meis2 and OP9-GFP co-culture, D7, treated with dox |
| GSM850833 |
CD41-, A2lox.Meis2 and OP9-GFP co-culture, D8, untreated |
| GSM850834 |
CD41int, A2lox.Meis2 and OP9-GFP co-culture, D8, untreated |
| GSM850835 |
CD41hi, A2lox.Meis2 and OP9-GFP co-culture, D8, untreated |
| GSM850836 |
CD41-, A2lox.Meis2 and OP9-GFP co-culture, D8, treated with dox |
| GSM850837 |
CD41int, A2lox.Meis2 and OP9-GFP co-culture, D8, treated with dox |
| GSM850838 |
CD41hi, A2lox.Meis2 and OP9-GFP co-culture, D8, treated with dox |
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| This SubSeries is part of SuperSeries: |
| GSE34583 |
Dual actions of Meis1 inhibit erythroid progenitor development and sustain general hematopoietic cell proliferation |
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| Relations |
| BioProject |
PRJNA156429 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34541_RAW.tar |
32.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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