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| Status |
Public on Mar 21, 2012 |
| Title |
Analysis of differential genetic expression by dietary intervention using moderate-carbohydrate restriction diet supplemented with calorie restriction mimetic multiple phytochemicals extracts(MCDmp) in B16F10 xenografted mouse model. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
We performed survival analysis of control and MCDmp groups, and explored underlying tumor suppression mechanisms after dietary intervention, focused on alterations in the energy-dependent signaling pathways, and global gene expression differences on cDNA microarray study, or histone modification on ChIP-on-chip analysis.
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| Overall design |
Illumina arrays: Five- week- old male C57BL6 mice were randomly divided into two groups and fed control diet (control group, LabDiet, Brentwood, MO, USA) or MCDmp formula (Treat group) in a specific pathogen free zone. All procedures were approved by the institutional animal use and care committee. Following a preliminary feeding of each diet formula for one week, 1 × 106 B16F10 cells (suspended with 100 μl of PBS) were subcutaneously injected into the back of the mice. After 24 days of diet supplementation, all mice were sacrificed. Tumor tissue was excised for cDNA microarray.
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| Contributor(s) |
Lee JD, Choi M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 20, 2012 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Jong-Doo Lee |
| E-mail(s) |
[email protected]
|
| Phone |
82-10-2829-1159
|
| Fax |
82-2-312-0578
|
| Organization name |
Yonsei University
|
| Street address |
250 seonsan-ro seodaemum-gu
|
| City |
Seoul |
| ZIP/Postal code |
120-752 |
| Country |
South Korea |
| |
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| Platforms (2) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
| GPL7099 |
Agilent-015279 Mouse CpG Island ChIP-on-Chip Microarray 2x105K (Feature Number version) |
|
| Samples (19)
|
| GSM897746 |
Control Diet Supplemented Mouse 4 |
| GSM897747 |
Control Diet Supplemented Mouse 5 |
| GSM897748 |
Control Diet Supplemented Mouse 6 |
| GSM897749 |
Control Diet Supplemented Mouse 7 |
| GSM897750 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 1 |
| GSM897751 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 2 |
| GSM897752 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 3 |
| GSM897753 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 4 |
| GSM897754 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 5 |
| GSM897755 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 6 |
| GSM897756 |
Control Diet Supplemented Mouse 1 |
| GSM897757 |
Control Diet Supplemented Mouse 2 |
| GSM897758 |
Control Diet Supplemented Mouse 3 |
| GSM897759 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 1 |
| GSM897760 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 2 |
| GSM897761 |
Moderate Restricted Carbohydrate Diet Supplemented Mouse 3 |
|
| Relations |
| BioProject |
PRJNA153631 |
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