|
| Status |
Public on Dec 05, 2012 |
| Title |
Neutrophil-derived IL-1β is sufficient for abscess formation in immunity against Staphylococcus aureus in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Neutrophil abscess formation is critical in innate immunity against many pathogens. Here, the mechanism of neutrophil abscess formation was investigated using a mouse model of Staphylococcus aureus cutaneous infection. Gene expression analysis of S. aureus-infected skin revealed that induction of neutrophil recruitment genes was largely dependent upon IL-1beta/IL-1R activation. Unexpectedly, using IL 1beta reporter mice, neutrophils were identified as the primary source of IL-1beta at the site of infection. Furthermore, IL-1beta-producing neutrophils were necessary and sufficient for abscess formation and bacterial clearance. S. aureus-induced IL 1beta production by neutrophils required TLR2, NOD2, FPRs and the ASC/NLRP3 inflammasome. Taken together, IL-1beta and neutrophil abscess formation during an infection are functionally, spatially and temporally linked as a consequence of direct IL-1beta production by neutrophils.
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| Overall design |
Lesional skin biopsies obtained from C57BL/6J WT mice or IL-1R-deficient mice at 4 hours post-infection with Staphylococcus aureus. Uninfected skin biopsies were also collected from WT and IL-1R-deficient mice.
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| |
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| Contributor(s) |
Cho JS, Miller LS |
| Citation(s) |
23209417 |
| |
| Submission date |
Mar 26, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Lloyd Miller |
| E-mail(s) |
[email protected]
|
| Phone |
(310) 206-6174
|
| Fax |
(310) 206-9878
|
| Organization name |
UCLA
|
| Department |
Medicine, Orthopaedic Surgery
|
| Street address |
52-121 Center for Health Sciences, 10833 Le Conte Avenue
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (12)
|
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| Relations |
| BioProject |
PRJNA156821 |
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