|
| Status |
Public on Sep 18, 2012 |
| Title |
Genome-wide mapping of human DNA-replication origins: levels of transcription at Orc1 sites regulate origin selection and replication timing |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the genome-wide mapping of Orc1 binding-sites in mammals and their validation as active DNA-replication origins (ORIs). Orc1 sites are universally associated with transcription start sites (TSSs) of coding or non-coding RNAs. Transcription levels at the Orc1 sites directly correlate with replication timing, suggesting the existence of two classes of ORIs: those associated with moderate/high transcription levels (≥1 RNA copy/cell), replicating in early S and mapping to the TSSs of coding RNAs, and those associated with low transcription levels (<1 RNA copy/cell), replicating throughout the entire S and mapping to TSSs of non-coding RNAs. These findings are compatible with a scenario whereby TSS expression-levels influence the efficiency of Orc1 recruitment at G1 and the probability of firing during S.
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| |
|
| Overall design |
Identification of Orc1 binding sites in human cells
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| |
|
| Contributor(s) |
Dellino GI, Cittaro D, Piccioni R, Luzi L, Banfi S, Segalla S, Cesaroni M, Giacca M, Pelicci PG |
| Citation(s) |
23187890 |
| |
| Submission date |
Apr 25, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Lucilla Luzi |
| Fax |
+390294375991
|
| Organization name |
IFOM FIRC Institute for Molecular Oncology Foundation
|
| Street address |
Via Adamello 16
|
| City |
Milano |
| ZIP/Postal code |
20139 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (13)
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| Relations |
| BioProject |
PRJNA162295 |
| SRA |
SRP012549 |
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