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| Status |
Public on Jun 20, 2013 |
| Title |
The APSES Transcription factor Efg1 regulates a novel phenotype switch in Candida parapsilosis [ChIP-seq]. |
| Organism |
Candida parapsilosis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We use ChIP-seq to identify the targets of Efg1 in Candida parapsilosis. We show that Efg1 binds to 502 promoter regions, including 70 potential transcription factors or regulatory proteins. Several of the transcription factors belong to networks that regulate biofilm development and white-opaque switching in C. albicans. Efg1 also binds to its own promoter. The binding site for C. parapsilosis Efg1 resembles that of orthologs in other fungi. Many Efg1 targets are probably also regulated by the Ndt80 transcription factor.
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| Overall design |
Efg1 in C. parapsilosis was tagged in situ using a myc epitope. Examination of Efg1-myc DNA binding sites by ChIP-seq. Three immunoprecipitated samples were sequenced and three input samples (pre-immunoprecipitation) were sequenced as controls.
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| Contributor(s) |
Connolly L, Riccombeni A, Lynch D, Holland L, Butler G |
| Citation(s) |
23895281 |
| |
| Submission date |
Sep 21, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Geraldine Butler |
| E-mail(s) |
[email protected]
|
| Organization name |
University Colege Dublin Conway Institute
|
| Department |
School of Biomolecular and Biomedical Science
|
| Lab |
Butler lab
|
| Street address |
Belfield
|
| City |
Dublin |
| ZIP/Postal code |
D4 |
| Country |
Ireland |
| |
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| Platforms (1) |
| GPL16091 |
Illumina Genome Analyzer IIx (Candida parapsilosis) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE41065 |
The APSES Transcription factor Efg1 regulates a novel phenotype switch in Candida parapsilosis. |
|
| Relations |
| BioProject |
PRJNA175661 |
| SRA |
SRP015839 |
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