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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 02, 2006 |
| Title |
Molecular Analysis of antigen-specific B cell responses |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In order to better understand the factors that regulate B cell differentiation upon exposure to antigen, we compares global gene expression profiles from naive B cells with antigen-specific plasma, germinal center, and memory B cells after immunization with the T-dependent antigen, NP-CGG. The memory B cell-enriched transcripts were then compared with memory T cell-enriched and hematopoietic stem cell-enriched transcripts in order to generate a transcriptional profile of self-renewal within the hematopoietic system.
Keywords: Cell Type Comparison
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| Overall design |
Naive B cells were FACS sorted from unimmunized mice, while plasma, germinal center, and memory B cells were sorted from NP-CGG-immunized mice at 1, 2, or 10 weeks after immunization, respectively, using a combination of cell surface markers. RNA was extracted, amplified, and hybridized to Affymetrix microarrays.
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| Contributor(s) |
Bhattacharya D, Weissman IL, Luckey CJ, Benoist C, Mathis D |
| Citation(s) |
16492737, 17982071 |
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| Submission date |
Jan 31, 2006 |
| Last update date |
Jul 31, 2019 |
| Contact name |
Irving L. Weissman |
| E-mail(s) |
[email protected], [email protected]
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| Phone |
6507235278
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| Fax |
6507234034
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| Organization name |
Stanford University
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| Street address |
279 Campus Dr.
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5323 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA94991 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE4142_RAW.tar |
80.2 Mb |
(http)(custom) |
TAR (of CEL) |
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