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Series GSE4324

Query DataSets for GSE4324

Status

Public on Jun 01, 2006

Title

Sex Differences in Response to Plasmodium chabaudi Infection: Involvement of Gonadal Steroids

Organism

Experiment type

Expression profiling by array

Summary

The goal of this study was to examine whether immune responses to Plasmodium chabaudi infection differ between the sexes and are altered by the presence of gonadal steroids. Gonadally-intact males were more likely than intact females to die following P. chabaudi infection, exhibit slower recovery from infection-associated weight loss, hypothermia, and anemia, have reduced IFNγ-associated gene expression and IFNγ production during peak parasitemia, and produce less antibody during the recovery phase of infection. Gonadectomy of male and female mice altered these sex-associated differences, suggesting that sex steroid hormone, in particular androgens and estrogens, may modulate immune responses to infection.
Keywords: Time Course

Overall design

Intact and gonadectomized (gdx) male and female C57BL/6 mice were inoculated with 106 P. chabaudi AS-infected erythrocytes and responses to infection were monitored. For microarray analyses, RNA was isolated from 5 x 106 white blood cells (WBCs) that were isolated from spleens 0, 3, 7, or 14 days after inoculation with P. chabaudi. Equivalent aliquots of RNA from 3 animals/treatment group were pooled and 3 separate pools were processed on separate Affymetrix GeneChips (n = 3 GeneChips/time point/treatment group).

Contributor(s)

Cernetich A, Garver LS, Jedlicka AE, Klein PW, Kumar N, Scott AL, Klein SL

Citation(s)

Submission date

Mar 02, 2006

Last update date

Jan 08, 2019

Contact name

Sabra Lynn Klein

E-mail(s)

[email protected]

Phone

(410) 955-8898

Fax

(410) 955-0105

Organization name

Johns Hopkins Bloomberg School of Public Health

Department

Molecular Microbiology and Immunology

Lab

Lab of Sabra Klein

Street address

615 North Wolfe Street

City

Baltimore

State/province

MD

ZIP/Postal code

21205

Country

USA

Platforms (1)

[MOE430A] Affymetrix Mouse Expression 430A Array

Samples (48)

More...

GSM98652	intactfemale_day0_control_rep01
GSM98653	intactfemale_day3_P.chabaudi_infected_rep01
GSM98654	intactfemale_day7_P.chabaudi_infected_rep01

GSM98655	intactfemale_day14_P.chabaudi_infected_rep01
GSM98656	gdxfemale_day0_control_rep01
GSM98657	gdxfemale_day3_P.chabaudi_infected_rep01
GSM98658	gdxfemale_day7_P.chabaudi_infected_rep01
GSM98659	gdxfemale_day14_P.chabaudi_infected_rep01
GSM98660	intactmale_day0_control_rep01
GSM98661	intactmale_day3_P.chabaudi_infected_rep01
GSM98662	intactmale_day7_P.chabaudi_infected_rep01
GSM98663	intactmale_day14_P.chabaudi_infected_rep01
GSM98664	gdxmale_day0_control_rep01
GSM98665	gdxmale_day3_P.chabaudi_infected_rep01
GSM98666	gdxmale_day7_P.chabaudi_infected_rep01
GSM98667	gdxmale_day14_P.chabaudi_infected_rep01
GSM98668	intactfemale_day0_control_rep02
GSM98669	intactfemale_day3_P.chabaudi_infected_rep02
GSM98670	intactfemale_day7_P.chabaudi_infected_rep02
GSM98671	intactfemale_day14_P.chabaudi_infected_rep02
GSM98672	gdxfemale_day0_control_rep02
GSM98673	gdxfemale_day3_P.chabaudi_infected_rep02
GSM98674	gdxfemale_day7_P.chabaudi_infected_rep02
GSM98675	gdxfemale_day14_P.chabaudi_infected_rep02
GSM98676	intactmale_day0_control_rep02
GSM98677	intactmale_day3_P.chabaudi_infected_rep02
GSM98678	intactmale_day7_P.chabaudi_infected_rep02
GSM98679	intactmale_day14_P.chabaudi_infected_rep02
GSM98680	gdxmale_day0_control_rep02
GSM98681	gdxmale_day3_P.chabaudi_infected_rep02
GSM98682	gdxmale_day7_P.chabaudi_infected_rep02
GSM98683	gdxmale_day14_P.chabaudi_infected_rep02
GSM98684	intactfemale_day0_control_rep03
GSM98685	intactfemale_day3_P.chabaudi_infected_rep03
GSM98686	intactfemale_day7_P.chabaudi_infected_rep03
GSM98687	intactfemale_day14_P.chabaudi_infected_rep03
GSM98688	gdxfemale_day0_control_rep03
GSM98689	gdxfemale_day3_P.chabaudi_infected_rep03
GSM98690	gdxfemale_day7_P.chabaudi_infected_rep03
GSM98691	gdxfemale_day14_P.chabaudi_infected_rep03
GSM98692	intactmale_day0_control_rep03
GSM98693	intactmale_day3_P.chabaudi_infected_rep03
GSM98694	intactmale_day7_P.chabaudi_infected_rep03
GSM98695	intactmale_day14_P.chabaudi_infected_rep03
GSM98696	gdxmale_day0_control_rep03
GSM98697	gdxmale_day3_P.chabaudi_infected_rep03
GSM98698	gdxmale_day7_P.chabaudi_infected_rep03
GSM98699	gdxmale_day14_P.chabaudi_infected_rep03

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE4324_RAW.tar	157.6 Mb	(http)(custom)	TAR (of CEL)

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