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| Status |
Public on Dec 31, 2013 |
| Title |
Genes regulated by ectopic expression of Sage in the entire Drosophila embryo using RNA from tub-Gal4 embryos. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
FoxA transcription factors play major roles in organ-specific gene expression. How FoxA proteins achieve specificity is unclear, given their broad expression patterns and requirements in multiple cell types. Here, we characterize Sage, a basic helix-loop-helix (bHLH) transcription factor expressed exclusively in the Drosophila salivary gland (SG). We identify Sage targets and show that not only are both Sage and the single Drosophila FoxA protein, Fork head (Fkh), required for expression of these genes, but coexpression of Sage and Fkh is sufficient to drive target gene expression in multiple other cell types. Sage and Fkh drive expression of the bZip transcription factor Senseless (Sens), which boosts expression of Sage/Fkh targets. Importantly, Sage, Fkh and Sens colocalize on salivary gland polytene chromosomes. Thus, Fkh drives cell-type specific gene expression as part of a tissue-specific transcription module that includes Sage and Sens, providing a new paradigm for how mammalian FoxA proteins acheive specificity.
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| Overall design |
Three control samples were obtained from the tub-Gal4 strain, age matched and treated the same as the three experimental samples isolated from Stages 11-16 embryos expressing UAS-Sage controlled by the tub-Gal4 driver.
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| Contributor(s) |
Fox RM, Andrew DJ |
| Citation missing |
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| Submission date |
Feb 05, 2013 |
| Last update date |
May 04, 2018 |
| Contact name |
Deborah J Andrew |
| E-mail(s) |
[email protected]
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| Organization name |
Johns Hopkins University School of Medicine
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| Department |
Cell Biology
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| Street address |
725 N. Wolfe St., Hunterian G-1
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA188563 |
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