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| Status |
Public on May 31, 2006 |
| Title |
Expression profiling of Treg cell versus their controls in human and mouse |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We applied Affymetrix GeneChip technology for expression profiling of CD4+CD25+ (Treg) T cells versus their naive CD4+CD25- controls to obtain more information about the underlying molecular mechanisms involved in Treg specific immune suppression
Keywords: cell type comparison
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| Overall design |
Expression profiles from:
[1] human Treg cells obtained from peripheral blood by cell sorting were compared to their naive controls. RNAs were prepared from pools of ten individual healthy donors, labeled in two independend labeling reactions and hybridized onto two GeneChips of the same lot.
[2] mouse Treg cells obtained from spleen by cell sorting of Balb/c mice were compared to their naive controls. RNAs were prepared from pools of three individual Balb/c mice, labeled in three independend labeling reactions and hybridized onto three GeneChips of the same lot.
The differences in gene expression were used to unravel molecular mechanisms that help to explain the suppressive and anergic phenotypes of Treg cells after antigen activation.
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| Contributor(s) |
Geffers R, Hansen W, Probst-Kepper M, Buer J |
| Citation(s) |
16836768 |
| |
| Submission date |
Mar 29, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Robert Geffers |
| E-mail(s) |
[email protected]
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| Phone |
+49 531-6181-3058
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| Organization name |
HCI - Helmholtz Centre for Infection Research
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| Department |
Dep. Molecular Bacteriology
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| Lab |
Genome Analytics
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| Street address |
Inhoffenstr. 7
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| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
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| Platforms (3) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL97 |
[HG-U133B] Affymetrix Human Genome U133B Array |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA94405 |
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