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| Status |
Public on Jun 07, 2014 |
| Title |
Quantitative transcriptome analysis of a high-GSH producing Saccharomyces cerevisiae and the wild type based on the second generation sequencing. |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The mutant Saccharomyces cerevisiae Y518 generated much more intracellular glutathione (GSH) than its counterpart dose. The RNA-seq based global transcriptome analysis was performed for exploring the potential mechanisms. Statistical analysis indicated that 1125 differentially expressed genes (fold-change>=2.0, FDR<=0.001) were up-regulated and 503 were down-regulated. There were 12 genes involved in glutathione metabolism. Of which GSH1, encodes gamma-glutamine cysteine synthetase, the rate-limiting enzyme in GSH biosynthesis process, was up-regulated. And MET17, encodes cysteine synthase A, an enzyme catalyzes the biosynthesis of one of the precursor amino acids L- cysteine, was also up-regulated. Besides, regulator SKN7 and several genes involved in oxidative stress response (GPX2, CTT1, SOD1, TRX2) were up-regulated. Intracellular ROS level of Y518 was also enhanced compared to that of 2-10515. Our results indicate that up-regulations of GSH1 and MET17 might be associated with the increased intracellular GSH content of the mutant, and up-regulated GSH1 may be caused by increased intracellular ROS level.
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| Overall design |
Saccharomyces cerevisiae mRNA profiles of 24-h wild type 2-10515 and mutant Y518 were generated by deep sequencing using Illumina HiSeqTM 2000
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| Contributor(s) |
Bin Q, Yibo Z, Limei W, Jiang S |
| Citation missing |
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| Submission date |
Jun 10, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiang Sun |
| E-mail(s) |
[email protected]
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| Organization name |
Changshu Institute of Technology
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| Street address |
No. 99, South Third Ring Road
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| City |
Changshu |
| ZIP/Postal code |
215500 |
| Country |
China |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA207805 |
| SRA |
SRP024391 |
