 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 01, 2013 |
| Title |
Saccharomyces cerevisiae promoter library |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
Combinatorial promoter expression level estimation via cell sorting
The purpose of this experiment was to determine the expression level of a library of synthetic promoters. The promoters were cloned in front of a GFP reporter and the resulting library transformed into yeast, sorted by FACS into six fluorescence bins, and the contents of the bins sequenced to determine the distribution of each promoter among each fluorescence bin. This was then used to calculate an expression level for each promoter with enough data.
|
| |
|
| Overall design |
The promoters were sorted into six bins and these, along with the unsorted library were barcoded and sequenced on a single lane of an Illumina HiSeq.
The following Series supplementary files are provided:
allPromoters.fsa.txt: the sequences of the promoters corresponding to the names in allPromoters.txt, not actually fasta format. Promoter sequence starts at pos 155 (0 indexed).
allPromoters.txt: the names of all the promoters, corresponding to the sequences in allPromoters.fsa.txt
barcodes.txt: the sequncing barcodes, corresponding to read2 from the sequencing files.
|
| |
|
| Contributor(s) |
de Boer CG, van Bakel H, Tsui K, Li J, Morris QD, Nislow C, Greenblatt JF, Hughes TR |
| Citation(s) |
24170600 |
| BioProject |
PRJNA200900 |
| |
| Submission date |
Jul 15, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Carl G de Boer |
| Organization name |
The Broad Institute
|
| Lab |
Aviv Regev
|
| Street address |
415 Main St
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (1) |
|
| Relations |
| SRA |
SRP021943 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48860_RAW.tar |
1.2 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE48860_allPromoters.fsa.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| GSE48860_allPromoters.txt.gz |
177.6 Kb |
(ftp)(http) |
TXT |
| GSE48860_barcodes.txt.gz |
90 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
