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| Status |
Public on Dec 31, 2013 |
| Title |
Transcriptional Response to transgenic BRAF(V600K) in the intestinal epithelium |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have analysed transcriptional effects of BRAF(V600K) in the intestinal epithelium of transgenic mice that harbour an inducible BRAF(V600K) transgene.
Mice used in this experiment were compound transgenic for a stem-cell specific Lgr5-EGFP Reporter and either an inducible TdTomato-2A-BRAF(V600K) transgene or an inducibe TdTomato-luciferase transgene.
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| Overall design |
Transgenes were induced by doxycycline (4mg/ml) treatment provided in a 1% sucrose solution in the drinking water of transgenic mice for 24h. Intestinal crypts were isolated by filtering (70um) following a PBS/EDTA incubation step. Single cell suspensions were made by digestion of crypts in 500ug/ml trypsine and 0.8u/ml DNAseI for approx. 20min at RT. Cell populations for profiling were isolated by FACS, using an Aria SOPR (BD), equipped with a 70um nozzle.
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| Contributor(s) |
Morkel M, Riemer P |
| Citation(s) |
25109331 |
| |
| Submission date |
Sep 06, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Markus Morkel |
| E-mail(s) |
[email protected]
|
| Organization name |
Charité - Universitätsmedizin Berlin
|
| Department |
Dept. of Pathology
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| Lab |
Laboratory of Molecular Tumour Pathology
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| Street address |
Charitéplatz 1
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA218462 |
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