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| Status |
Public on Dec 01, 2014 |
| Title |
Assessing inter- and intra-individual DNA methylation profiles in human brain samples |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
We report the application of single molecule-based sequencing technology in combination with MBD affinity purifcation (MAP-seq) to generate methylation maps in a panel of human brain samples. Here we assay the methylation status of the human genome in eight distinct brain regions from three individuals in addition to a further thirty six hippocampal samples. The cerebellum was a consistent outlier compared to all other regions, showing over 16,000 differentially methylated regions (DMRs). Unexpectedly, the sequence properties of hypo- and hyper- methylated domains in cerebellum were distinct. In contrast we found very few DMRs that could distinguish between regions of the cortex, limbic system and brain stem. Inter-individual DMRs were readily detectable, however with the exception of cerebellum, DNA methylation patterns are more similar between different brain regions from the same individual, than they are for a single brain region between different individuals.
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| |
|
| Overall design |
Examination of DNA methylation status in human brain samples.
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| |
|
| Contributor(s) |
Illingworth RS, Gruenewald-Schneider U, DeSousa D, Webb S, Merusi C, Kerr AR, James KD, Smith C, Walker R, Andrews RM, Bird AP |
| Citation(s) |
25572316 |
| |
| Submission date |
Sep 18, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Rob S Illingworth |
| E-mail(s) |
[email protected]
|
| Phone |
01316519640
|
| Organization name |
The University of Edinburgh
|
| Department |
Centre for regenerative Medicine
|
| Lab |
Illingworth
|
| Street address |
Centre for Regenerative Medicine
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH16 4UU |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (59)
|
|
| Relations |
| BioProject |
PRJNA219443 |
| SRA |
SRP029999 |
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