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Series GSE52085 Query DataSets for GSE52085
Status Public on Mar 07, 2014
Title Liver functional genomics in beef cows on grazing systems
Organism Bos taurus
Experiment type Expression profiling by array
Summary We evaluated liver transcriptome throughout gestation and early lactation of purebred and crossbred beef cows (Angus, Hereford and their F1 crossbreeds, CR), grazing high or low herbage allowances (HA) of native grasslands (4 and 2.5 kg DM/kg BW annual mean; n = 16) using an Agilent 4x44k bovine array. A total of 4,661 transcripts were affected by days (272 ≥ 2.5-fold difference, FDR ≤ 0.10) and 47 pathways were altered during winter gestation (-165 to -15 days relative to calving, d). Gluconeogenesis and fatty acid oxidation pathways were substantially upregulated, while cell growth, DNA replication and transcription pathways were downregulated (FDR ≤ 0.25). We observed only small changes in the liver transcriptome during the early lactation period (+15 to +60 d). A total of 225 genes were differentially expressed (47 ≥ 2-fold difference, FDR ≤ 0.10) between HA. The majority of those were related to glucose and pyruvate metabolism and were upregulated in high HA, reflecting their better metabolic status. Two genes were upregulated in CR cows, but 148 transcripts (74 ≥ 2-fold change difference, FDR ≤ 0.10) were affected by the HA and cow genotype interaction. The transcriptional changes observed indicates a complex and previously unrecognized adaptive program in beef cow liver. We report novel candidate genes, metabolic pathways, and regulatory mechanisms, which can be used as targets of future studies.
 
Overall design Complete randomized block design with two spatial replications and a factorial arrangement of herbage allowance (HA) and cow genotype (CG). Herbage allowance treatments represented 4 and 2.5 kg DM/kg BW of annual mean (HI and LO, respectively). Four purebred (PU – Hereford, H and Angus, A) and four crossbred (CR - F1, HA and AH) multiparous pregnant cows (5 to 6 year-old), were evaluated per treatment (HI-CR, HI-PU, LO-CR, and LO-PU) throughout the gestation and lactation period (-165 to +60 days relative to parturition, days). Cows belonged to or were descendants of experimental animals generated as part of a diallel crossbreeding experiment carried out for 10 years at the Experimental Station and were assigned to the same forage allowance (HI or LO) since May 2007 and gestated and lactated one calf every year from 2007 to 2009.
 
Contributor(s) Carriquiry M, Laporta J, Naya H, Rosa GJ
Citation(s) 24326346
Submission date Nov 05, 2013
Last update date Mar 07, 2014
Contact name Mariana Carriquiry
E-mail(s) [email protected]
Phone +59823572344
Organization name Facultad de Agronomía, UdelaR
Department Department of Animal Sciences and Pastures
Street address Ave Garzon 780
City Montevideo
ZIP/Postal code 12300
Country Uruguay
 
Platforms (1)
GPL11648 Agilent-023647 B. taurus (Bovine) Oligo Microarray v2 (Feature Number version)
Samples (64)
GSM1259130 Liver_high forage allowance_crossbred_-165 days_replicate 1
GSM1259131 Liver_high forage allowance_crossbred_-15 days_replicate 1
GSM1259132 Liver_high forage allowance_crossbred_+15 days_replicate 1
Relations
BioProject PRJNA227132

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52085_RAW.tar 450.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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