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| Status |
Public on Jan 01, 2015 |
| Title |
Transcript profiling in stem base of crown root less 1 mutant after ectopic expression induction by dexamethasome of CRL1 |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
Lateral Organ Boundary Domain (LBD) transcription factors are specific of plants and are involved in the control of development. One LBD clade is related to the control of root development (Coudert et al., 2013, Mol. Biol. Evol. 30, 569-572). Belonging to this clade, CROWN ROOT LESS 1 controls the initiation of crown roots in rice (Inukai Plant Cell, 17, 1387-1396, Liu et al., 2005, Plant J., 43, 47-56). The aim of this study was to identify the genes that are regulated by CRL1.
Transcriptome analysis in crl1 mutant plants where the CRL1 gene expression was ectopically induced revealed a set of 277 up regulated genes including a large part of genes encoding transcription factors and genes encoding proteins associated to hormone homeostasis, to cell division, expansion and differentiation.
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| Overall design |
We transformed crl1 mutant plants with a construction allowing the control of CRL1 expression by exogenous dexamethasone. Three independents transgenic lines were used. Controls were untranformed or transformed with an empty plasmid crl1 mutant plants. Transcriptome profiling were conducted 4h and 8h after dexamethasone treatment to identify early CRL1-activated genes.
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| Contributor(s) |
Coudert Y, Gantet P |
| Citation(s) |
25442012 |
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| Submission date |
Dec 12, 2013 |
| Last update date |
Dec 27, 2017 |
| Contact name |
Pascal Gantet |
| E-mail(s) |
[email protected]
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| Organization name |
Université Montpellier 2
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| Department |
IRD
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| Lab |
UMR DIADE
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| Street address |
Avenue Agropolis
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| City |
Montpellier |
| ZIP/Postal code |
34000 |
| Country |
France |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (20)
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| GSM1288794 |
control line 1, crl1 mutant, 4 hours after mock treatment |
| GSM1288795 |
control line 1, crl1 mutant, 4 hours after dexamethasone treatment |
| GSM1288796 |
control line 1, crl1 mutant, 8 hours after mock treatment |
| GSM1288797 |
control line 1, crl1 mutant, 8 hours after dexamethasone treatment |
| GSM1288798 |
control line 2, crl1 mutant transformed with empty pINDEX2, 4 hours after mock treatment |
| GSM1288799 |
control line 2, crl1 mutant transformed with empty pINDEX2, 4 hours after dexamethasone treatment |
| GSM1288800 |
control line 2, crl1 mutant transformed with empty pINDEX2, 8 hours after mock treatment |
| GSM1288801 |
control line 2, crl1 mutant transformed with empty pINDEX2, 8 hours after dexamethasone treatment |
| GSM1288802 |
DEX::CRL1 line 1, crl1 mutant transformed with pINDEX2-CRL1, 4 hours after mock treatment |
| GSM1288803 |
DEX::CRL1 line 1, crl1 mutant transformed with pINDEX2-CRL1, 4 hours after dexamethasone treatment |
| GSM1288804 |
DEX::CRL1 line 1, crl1 mutant transformed with pINDEX2-CRL1, 8 hours after mock treatment |
| GSM1288805 |
DEX::CRL1 line 1, crl1 mutant transformed with pINDEX2-CRL1, 8 hours after dexamethasone treatment |
| GSM1288806 |
DEX::CRL1 line 2, crl1 mutant transformed with pINDEX2-CRL1, 4 hours after mock treatment |
| GSM1288807 |
DEX::CRL1 line 2, crl1 mutant transformed with pINDEX2-CRL1, 4 hours after dexamethasone treatment |
| GSM1288808 |
DEX::CRL1 line 2, crl1 mutant transformed with pINDEX2-CRL1, 8 hours after mock treatment |
| GSM1288809 |
DEX::CRL1 line 2, crl1 mutant transformed with pINDEX2-CRL1, 8 hours after dexamethasone treatment |
| GSM1288810 |
DEX::CRL1 line 3, crl1 mutant transformed with pINDEX2-CRL1, 4 hours after mock treatment |
| GSM1288811 |
DEX::CRL1 line 3, crl1 mutant transformed with pINDEX2-CRL1, 4 hours after dexamethasone treatment |
| GSM1288812 |
DEX::CRL1 line 3, crl1 mutant transformed with pINDEX2-CRL1, 8 hours after mock treatment |
| GSM1288813 |
DEX::CRL1 line 3, crl1 mutant transformed with pINDEX2-CRL1, 8 hours after dexamethasone treatment |
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| Relations |
| BioProject |
PRJNA231520 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53272_RAW.tar |
85.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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