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Series GSE5593 Query DataSets for GSE5593
Status Public on Sep 19, 2007
Title Acetaminophen (APAP) Rat Blood Training Gene Expression Data Set
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Gene expression training data set from rat blood samples exposed to either 150, 1500 or 2500 mg/kg of APAP for 6, 12 or 24 hours.
Keywords: Dose response, Time course, Microarray, Gene expression
 
Overall design Male F344/N rats, 10-12 weeks old, were exposed to 0 (vehicle only), 150, 1500 or 2500 mg/kg APAP in 0.5% ethyl cellulose by oral gavage in two doses to increase absorption. The animals were sacrificed after 6, 12 or 24 hours. Equal amounts of RNA from the blood from each of four vehicle-only treated animals at the 6 and 12 hour time point and from each of six vehicle-only treated animals at the 24 hour time point, were pooled for control gene expression. The pooled controls were compared with individual treated animals at each dose and time period. The samples were hybridized in duplicate for each individual rat for a total of 8 (exception due to insufficient RNA quality: 150 mg/kg APAP and 1500 mg/kg APAP at 12 hour time point only 3 animals) microarray chips per dose and time period.

Experiments were performed according to the guidelines established in the NIH Guide for the Care and Use of Laboratory Animals and an approved Animal Study Protocol was on file prior to initiation of the study.
 
Contributor(s) Bushel PR, Heinloth AN, Paules RS
Citation(s) 17984051
Submission date Aug 24, 2006
Last update date Dec 06, 2012
Contact name Pierre Robert Bushel
E-mail(s) [email protected]
Phone 919-316-4564
Organization name NIEHS
Department Biostatistics and Computational Biology Branch
Lab Bioinformatics
Street address P.O. Box 12233
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platforms (1)
GPL890 Agilent-011868 Rat Oligo Microarray G4130A (Feature Number version)
Samples (68)
GSM129829 3616 PC 24Hr (pool) vs. 150mg/kg APAP 24Hr rat 3617 blood
GSM129830 3616 PC 24Hr (pool) vs. 150mg/kg APAP 24Hr rat 3618 blood
GSM129831 150mg/kg APAP 24Hr rat 3617 blood vs. 3616 PC 24Hr (pool)
This SubSeries is part of SuperSeries:
GSE5652 Blood and liver exposed to acetaminophen (APAP)
Relations
BioProject PRJNA104357

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5593_RAW.tar 2.8 Mb (http)(custom) TAR

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