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| Status |
Public on Jul 03, 2014 |
| Title |
Dose-dependent hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to sublethal doses of gamma radiation |
| Platform organism |
salmonid fish |
| Sample organism |
Salmo salar |
| Experiment type |
Expression profiling by array
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| Summary |
To study the short term (48 h) hepatic transcriptional changes and identify potential modes of action, Atlantic salmon (Salmo salar) were exposed to 15 mGy, 70 mGy and 280 mGy external gamma radiation. A combination of high density (60 k) custom oligonucleotide salmonid microarray and quantitative real-time reverse transcription polymerase chain reaction (qPCR) was employed to perform gene expression analyses. Differentially expressed genes (DEGs) were determined using one-way analysis of variance (ANOVA) and Tukey posthoc tests. Functional enrichment analysis based on Gene Ontology (GO) was performed to link DEGs to their biological functions. The Salmo salar DEGs were further mapped to mammlian orthologs. By using ortholog DEGs, gene networks were built based on well-curated mammlian protein-protein interactions and pathways analyses were performed to link DEGs to specific toxicological/biological functions. The results obtained from microarray analysis were further verified using qPCR.
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| Overall design |
Juvenile Atlantic salmon were exposed to external gamma radiation emitted from a cobalt-60 source for 48 h. Liver were sampled and used for gene expression analysis.
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| Contributor(s) |
Song Y, Salbu B, Teien H, Heier L, Rosseland B, Tollefsen K |
| Citation(s) |
25146236 |
| |
| Submission date |
Jul 02, 2014 |
| Last update date |
Sep 01, 2014 |
| Contact name |
You Song |
| E-mail(s) |
[email protected]
|
| Organization name |
Norwegian Institute for Water Research (NIVA)
|
| Department |
Ecotoxicology and Risk Assessment
|
| Street address |
Økernveien 94
|
| City |
Oslo |
| ZIP/Postal code |
0579 |
| Country |
Norway |
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| Platforms (1) |
| GPL18864 |
Agilent-035370 Salmonid_60k_Array_V1_060711 035285 |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA254169 |
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