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Series GSE61675 Query DataSets for GSE61675
Status Public on Sep 24, 2014
Title Transcriptome of Arabidopsis thaliana ceh1 mutant
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Here we present the transcriptomic profile of mutant plants designated as ceh1 (constitutively expressing HPL). CEH1 encodes 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase (HDS), the enzyme controlling the bottleneck step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the plastids. Mutation of this enzyme in ceh1 mutant led to accumulation of high levels of the stress specific signaling metabolite 2c-methyl-D-erythritol 2,4-cylclodiphosphate (MEcPP), and consequently constitutive activation of a selected otherwise stress responsive genes. This data identifies the ensemble of stress responsive genes whose expression is regulated by the MEcPP signaling cascade.
 
Overall design About 20 two-week-old plants grown on 1/2 MS medium were collected to extract total RNA. Transcriptome of parent line and ceh1 mutant were compared.
 
Contributor(s) Xiao Y, Dehesh K
Citation(s) 25922532
Submission date Sep 23, 2014
Last update date Jun 12, 2017
Contact name Katayoon Dehesh
E-mail(s) [email protected]
Organization name UNIVERSITY OF CALIFORNIA DAVIS
Department Plant Biology
Lab 1203 Life Science Addition
Street address One Shilels Avenue
City Davis
State/province CA
ZIP/Postal code 95776
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (6)
GSM1511121 Parent line, biological rep1
GSM1511122 Parent line, biological rep2
GSM1511123 Parent line, biological rep3
Relations
BioProject PRJNA261814

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61675_RAW.tar 13.1 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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